# Core C: Analytical Core

> **NIH NIH P01** · NATHAN S. KLINE INSTITUTE FOR PSYCH RES · 2021 · $357,524

## Abstract

ABSTRACT – Core C
This PPG is a multidisciplinary investigation of in vivo and in vitro models of endosomal, autophagic, and
lysosomal abnormalities in Alzheimer's disease (AD) considered critical to AD pathogenesis. This Core
provides specialized services, required by at least three of the four Projects of the PPG. These services are
best coordinated by a core to increase efficiencies of time and cost, promote scientific synergies, and
maximally utilize dedicated highly specialized facilities. The specific aims of Core C are: (1) To acquire, store
and efficiently distribute well-characterized tissues (brains and fibroblasts) from cases of AD, FAD, Trisomy 21
and non-demented and non-AD neurological controls (e.g., PD, HD) in a coordinated manner for studies in the
PPG. Cultured primary mouse neurons and glial subtypes prepared under standardized conditions will be
distributed routinely. (2) To perform electron microscopy and immunogold electron microscopy on cell/mouse
models, human brains and isolated subcellular fractions including extracellular vesicles. (3) To perform ELISA
to quantify Aβ and other APP metablolites in AD brains, brains of mouse models and media and lysates of
fibroblasts and other cell models. (4) To provide specialized histological procedures, morphometric techniques,
fluorescence/confocal applications and neurosurgical procedures as needed for all four Projects as well as
provide training on laser confocal microscopy and video-fluorescent microscopy. (5). To maintain, distribute
and purify polyclonal and monoclonal antibodies generated in-house. (6) To administer to mouse models
behavioral assays of memory and motor performances from a highly standardized battery of validated tests. (7)
To coordinate collaborations between investigators in the PPG and in Columbia University and NYULMC
facilities for studies employing lipidomics, proteomics and RNA-sequencing technologies and maintain an
accessible bank for the acquired data. The Core also provides standardized instructions for reagents (e.g.,
antibodies), cell culture conditions, procedures/methods, data capture/analysis to ensure reproducibility for the
same method across the Projects. The Core serves all Projects and is essential to their success.

## Key facts

- **NIH application ID:** 10126775
- **Project number:** 5P01AG017617-20
- **Recipient organization:** NATHAN S. KLINE INSTITUTE FOR PSYCH RES
- **Principal Investigator:** RALPH A. NIXON
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $357,524
- **Award type:** 5
- **Project period:** 2000-02-15 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10126775

## Citation

> US National Institutes of Health, RePORTER application 10126775, Core C: Analytical Core (5P01AG017617-20). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10126775. Licensed CC0.

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