# Core A: Cell Analysis

> **NIH NIH P01** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 2021 · $213,464

## Abstract

PROJECT SUMMARY
The “Cell Analysis Core”, designated “Core A” in this application is part of the Program Project on “Processing
and Repair of DNA Crosslinks”. This core will provide a unified, quality controlled system of mammalian cell
analyses services for efficient operation of the Program Project. The program involves four integrated projects
where an essential element is the quantitative measurement of sensitivity to DNA damaging agents. It is
essential that these be performed in a unified and standardized way so that data can be compared within
projects and conveyed between them. Each project will use cell lines engineered by advanced methods for
disruption or depletion of specific genes. It is essential that each cell line is propagated under optimal
conditions, that the genetic identify of each cell is established beyond doubt, and that cells are free of
mycoplasma and other infections. Core A will perform the clonogenic survival assays that will be fundamental
to the success of all four Projects. Second, the Core will characterize, maintain quality control of, preserve and
distribute a large number of cell lines, which will allow the projects the core is supporting to progress more
rapidly. Finally, Core A will produce large-scale cultures of mammalian cells containing overexpressed protein
for transfer to Projects when needed for subsequent protein purification.

## Key facts

- **NIH application ID:** 10129290
- **Project number:** 5P01CA193124-05
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **Principal Investigator:** RICHARD D WOOD
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $213,464
- **Award type:** 5
- **Project period:** 2017-02-10 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10129290

## Citation

> US National Institutes of Health, RePORTER application 10129290, Core A: Cell Analysis (5P01CA193124-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10129290. Licensed CC0.

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