# In vivo model of microglia inflammasome signaling in neuroinflammation

> **NIH NIH R24** · IOWA STATE UNIVERSITY · 2020 · $294,484

## Abstract

Project Summary/Abstract
In this Administrative Supplement application, we propose to apply our targeted integration strategy to build
a novel in vivo zebrafish model of microglia inflammasome signaling in neuroinflammation. Chronic
neuroinflammation driven by microglia activation underlies the pathophysiology of Alzheimer’s Disease (AD)
and (PD) and related dementia. a-synuclein secreted by neurons in Parkinson’s patients form aggregates
that are taken up by microglia, however, our understanding of the downstream intracellular mechanisms
that signal microglia inflammasome activation is incomplete. We will build on our expertise in zebrafish site
directed genome engineering and mouse models of neuroinflammation to generate a zebrafish model of
conditional active fyn kinase that will allow temporal and spatial induction of microglia inflammasome
signaling and activation. Our model will provide an in vivo platform for phenotypic chemical screens that
recapitulates the cellular complexity of neuroinflammation. The new tools we develop for microglia specific
Cre expression and conditional control of inflammasome signaling will provide an important resource for
zebrafish researchers investigating immune function in disease pathogenesis as well as development of
translational strategies.

## Key facts

- **NIH application ID:** 10129553
- **Project number:** 3R24OD020166-05S1
- **Recipient organization:** IOWA STATE UNIVERSITY
- **Principal Investigator:** KARL J CLARK
- **Activity code:** R24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $294,484
- **Award type:** 3
- **Project period:** 2016-06-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10129553

## Citation

> US National Institutes of Health, RePORTER application 10129553, In vivo model of microglia inflammasome signaling in neuroinflammation (3R24OD020166-05S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10129553. Licensed CC0.

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