# Einstein Mount Sinai Diabetes Research Center

> **NIH NIH P30** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 2021 · $335,655

## Abstract

The goal of the Stable Isotope and Metabolomics Core (SIMC) is to provide to Einstein-Mount
Sinai Diabetes Research Center research investigators cost effective, efficient and robust
platforms for the determination of metabolites/lipids designed to cover interlocking metabolite
and lipid pathways, and help to improve our understanding of conditions which change fuel
utilization and biosynthesis in cytosolic and mitochondrial metabolic pathways, i.e., glycolysis,
pentose and tricarboxylic acid (TCA) cycles. To accomplish these goals in one facility, the SIMC
has updated its technology infrastructure to include the latest Seahorse XFe24 and XFe96
Seahorse Flux Analyzers for assessment of glycolytic and mitochondrial energetics in real time,
the latest Sciex 6500+ QTRAP with Selexion ion mobility for targeted LC/MS.MS metabolite and
lipidomic analyses, and is due to replace its Orbitrap/LTQ high mass accuracy instrument with
the latest Shimadzu LC-9030 quadrupole Time of Flight with Nexera UPLC for untargeted
metabolomic and lipidomic analyses. These latest additions add to the high mass resolution
Waters GC-TOF/MS and two unit Dalton resolution Agilent single quadrupole GC/MS machines
to accomplish the following aims:
1. To determine metabolomic/lipidomic profiles for the diagnosis/characterization of
 physiological and pathophysiological states in cells, tissues and biofluids (plasma, urine,
 stool, etc.).
2. To perform in vivo stable isotope substrate assays to determine rates of protein synthesis,
 lipogenesis, peripheral glucose disposal, hepatic glucose recycling, glucose-glycerol cycling
 and Cori cycling, high energy phosphate (ATP, creatine phosphate) turnover, and in vitro
 stable isotope flux dissections of metabolic pathways.
3. To perform assessments of glycolysis (extracellular acidification rates, glycolytic ATP
 production rates) and mitochondrial oxygen consumption (mitochondrial respiration and
 mitochondrial ATP production rates) in tissue explants, primary isolated and tissue culture
 cells using Seahorse Biosciences Flux Analyzers.
4. To advise and instruct ES-DRC investigators and their students, fellows and technical staff
 in the design and interpretation of fluxomics, metabolomics/lipidomics and computational
 analyses with the aim of elucidating the molecular basis underlying glucose and lipid
 homeostasis in humans and animal models.

## Key facts

- **NIH application ID:** 10130495
- **Project number:** 5P30DK020541-46
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Irwin Jack Kurland
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $335,655
- **Award type:** 5
- **Project period:** 1996-12-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10130495

## Citation

> US National Institutes of Health, RePORTER application 10130495, Einstein Mount Sinai Diabetes Research Center (5P30DK020541-46). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10130495. Licensed CC0.

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