# Deep Supercooling of Red Blood Cells: Towards Practical Long Term Storage

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 2021 · $377,785

## Abstract

Abstract
 Red blood cells (RBCs) are possibly the most transfused blood component and the most widely stored cell
type. While cold storage (+4 oC) of RBCs has been vastly improved in the last few decades with a standard
storage time of 42 days in clinical settings, recent clinical retrospective as well as laboratory studies indicate that
beyond 14 days of storage RBCs might have vastly different biochemical properties and possibly inferior
outcomes in patients. Here, we propose to develop an RBC preservation method based on our recent
breakthrough in deep supercooling (DSC) of aqueous solutions where we can achieve seemingly stable
supercooling for large volumes and at very low temperatures (down to -20 oC) for very long times. This is
achieved by surface sealing of aqueous solutions via water-immiscible hydrocarbon-based liquids. Our central
hypothesis, based on preliminary studies and prior work, is that DSC of RBCs can provide an immediately
practical, high quality, and long-term storage, as an alternative to current clinical standard of cold storage. Our
approach is to first establish a robust characterization framework for storage related injuries to a) establish the
basic temperature optimization of DSC for RBCs, b) while comparing the cell quality of this basic approach to
cold storage and cryopreservation. We will then supplement the DSC approach drawing from our experience in
alleviating lipid peroxidation, oxidative stress, and membrane injuries and metabolic suppression along with
recent advances in RBC preservation such as anaerobic preservation to achieve long-term storage of RBCs in
small volumes. Finally, we aim to extend the range of parameters for DSC to the 150-500 ml range (volume), ~-
25-30 oC (temperature), and 150 days (time) in parallel. Our final goal is to conduct storage with these robust
DSC strategies to preserve a clinical unit (~300 ml) of RBCs for 150 days. By completing this project, we expect,
to demonstrate a novel method to dramatically extend the storage time for RBCs to 150 days whereby alleviating
problems of current approaches. In the long-term the “large volume DSC” method, is widely applicable to all cell,
tissue and organs; especially those that are not amenable to cryopreservation. These advances will positively
influence healthcare by enabling storage of living matter for applications in cell/organ transplantation, engineered
tissue logistics, and food storage among others

## Key facts

- **NIH application ID:** 10130602
- **Project number:** 5R01HL145031-02
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** Osman Berk USTA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $377,785
- **Award type:** 5
- **Project period:** 2020-04-01 → 2024-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10130602

## Citation

> US National Institutes of Health, RePORTER application 10130602, Deep Supercooling of Red Blood Cells: Towards Practical Long Term Storage (5R01HL145031-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10130602. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*