# Design of Fe2+ and H2O2 Induced Proximity Functionalized Imaging Probes for the Control of Cellular Functions

> **NIH NIH R01** · UNIVERSITY OF ARIZONA · 2020 · $116,500

## Abstract

Project Summary:
 In this administrative supplement, we request funds for a new instrument, Molecular Devices ImageXpress
Micro 4 High Content Screening System. The instrument will allow for the measurement of both luminescence
and fluorescence of tailored fluorescent and chemiluminescent Fe(II) and H2O2 responsive chemical inducers in
chemical systems and in live cell and subcellular localization imaging and quantitative detection of luminescence
and fluorescence. In the funded 1R01GM130772 grant, we propose a novel synthetic biology technology termed
“Environment Stimuli-Induced Proximity (ESIP)” to spatiotemporally manipulate cellular functions. To
demonstrate the feasibility, we propose to develop fluorescent and chemiluminescent Fe(II) and H2O2
responsive ESIP chemical inducers. Specifically, we will design, synthesize, test and optimize Fe2+ and
H2O2-responsive fluorescent and chemiluminescent ESIP inducers (Specific Aims 1-2) and test them in cells and
construct ESIP-mediated “AND” Boolean logic gates to control the conditional production of AD therapeutic
proteins (Specific Aim 3). Therefore, this instrument is crucial for us to quickly and accurately characterize and
detect fluorescent and chemiluminescent Fe(II) and H2O2 responsive ESIP chemical inducers in chemical and
biological systems. This instrument will be the first at the University of Arizona, and will be available to all the
Principal Investigators who are currently funded by NIGMS and other mechanisms.

## Key facts

- **NIH application ID:** 10131340
- **Project number:** 3R01GM130772-02S1
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **Principal Investigator:** WEI WANG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $116,500
- **Award type:** 3
- **Project period:** 2019-05-01 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10131340

## Citation

> US National Institutes of Health, RePORTER application 10131340, Design of Fe2+ and H2O2 Induced Proximity Functionalized Imaging Probes for the Control of Cellular Functions (3R01GM130772-02S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10131340. Licensed CC0.

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