# Flow cytometry and cell sorting for structural biophysics in immune recognition

> **NIH NIH R35** · UNIVERSITY OF NOTRE DAME · 2020 · $211,853

## Abstract

Project Summary
Supplement funds are requested under PA-18-591 and NOT-GM-20-013 to purchase a BD FACS Melody cell
sorting instrument. The system will support NIGMS award R35GM118166. Research in this award emphasizes
the interface between structural biology, molecular biophysics, and immunology. Broadly speaking, work in this
project strives to connect the fundamental biophysical principles that govern protein behavior with molecular
recognition in the immune system. This work is also instructional for understanding the basic rules of molecular
recognition and elucidating principles for protein modeling and design in complex systems. Major amounts of
work in the project now relies on library screening. This is used to identify mutations which govern specificity, to
understand how specificity and affinity can be altered, and to identify which targets are recognized and which
are not. The screening processes rely on fluorescent activated cell sorting. Technology currently available to the
laboratory is aging, unnecessarily complex, requires advance scheduling, and necessitates paying expensive
user fees. The streamlined, single laboratory system requested here will permit more a rapid advance towards
the project goals. 85% of the system cost is requested from NIGMS. The remaining 15% will be contributed by
the University of Notre Dame.

## Key facts

- **NIH application ID:** 10131481
- **Project number:** 3R35GM118166-05S1
- **Recipient organization:** UNIVERSITY OF NOTRE DAME
- **Principal Investigator:** Brian M Baker
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $211,853
- **Award type:** 3
- **Project period:** 2016-05-01 → 2021-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10131481

## Citation

> US National Institutes of Health, RePORTER application 10131481, Flow cytometry and cell sorting for structural biophysics in immune recognition (3R35GM118166-05S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10131481. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*