# Project 2 - Host-virus networks regulating flu replication and host responses ex vivo

> **NIH NIH U19** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2020 · $364,291

## Abstract

The goal of our Fluomics Next Generation U19 program is to collect multidimensional systems biology
measurements in influenza virus infected cells, animals and humans to understand virus host-pathogen
interactions that underlie disease pathogenesis and provide novel targets for therapeutic intervention. As part
of the Fluomics U19 program, our labs have developed and implemented a pipeline consisting of high
throughput genome-wide screens to identify host factors involved in virus replication, followed by functional
validation in vivo in a mouse model of infection. This pipeline will be applied to rapidly respond to the
emergency caused by the recent human outbreak of a novel coronavirus known as SARS-CoV-2 that causes
in many infected patients a severe, sometimes lethal, respiratory disease named COVID-19.
Specifically, we will use our established high throughput screen tools to rapidly evaluate the potential antiviral
activity against SARS-CoV-2 of a chemical library, the ReFRAME collection, that includes nearly all small
molecules that have reached clinical development or have undergone significant profiling including optimized
pharmacokinetics, safety profiles, and annotations of human dosing. Compounds showing best antiviral
activities and known safety profiles will be evaluated in human ACE2 transgenic mice, known to support
SARS-CoV-2 replication. By the end of this project, we expect to have at least 1-2 compounds or combination
therapies that exhibit SARS-CoV-2 antiviral activity in vivo, which can be immediately moved into the clinic.

## Key facts

- **NIH application ID:** 10132158
- **Project number:** 3U19AI135972-03S1
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** SUMIT K CHANDA
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $364,291
- **Award type:** 3
- **Project period:** 2020-03-30 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10132158

## Citation

> US National Institutes of Health, RePORTER application 10132158, Project 2 - Host-virus networks regulating flu replication and host responses ex vivo (3U19AI135972-03S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10132158. Licensed CC0.

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