# Regulation of RNA Polymerase II transcription by the phosphatase Rtr1

> **NIH NIH R01** · INDIANA UNIVERSITY INDIANAPOLIS · 2020 · $68,562

## Abstract

Project Summary/Abstract
The control of RNA Polymerase elongation and termination is not fully understood. In this work, we will
investigate the role of the C-terminal domain phosphatases Rtr1 and Ssu72 in the regulation of RNA
Polymerase II (RNAPII) elongation and termination. Our preliminary findings suggest that deletion of Rtr1
results in global changes in the efficiency of early termination of RNAPII during transcription elongation.
Previous work has shown that disruption of Ssu72 function leads to decreased RNAPII termination resulting in
terminator read-through defects. In this proposal, we will test how perturbations of each phosphatase effect
transcription elongation and termination. Additionally, we will perform enzymatic characterization of Rtr1 and
Ssu72 substrate specificity to further characterize their mechanisms of action. Finally, we will determine the
degree of interplay between Rtr1 and Ssu72 to determine if they are able to target unique and/or overlapping
sites within the RNAPII C-termination domain. Overall, these studies will shed light on the fundamental process
of RNAPII transcription control which is disrupted in numerous disease states in humans.

## Key facts

- **NIH application ID:** 10132166
- **Project number:** 3R01GM099714-08S1
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** AMBER L. MOSLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $68,562
- **Award type:** 3
- **Project period:** 2012-08-01 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10132166

## Citation

> US National Institutes of Health, RePORTER application 10132166, Regulation of RNA Polymerase II transcription by the phosphatase Rtr1 (3R01GM099714-08S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10132166. Licensed CC0.

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