# Role of Bacteria in Colitis-Associated Colon Cancer

> **NIH NIH R01** · UNIVERSITY OF FLORIDA · 2022 · $429,377

## Abstract

Summary:
 During the last funding cycle, we demonstrated that specific microbial activities are important for
development of CRC, for example the presence of the pathogenic pks gene island, in certain E.coli of the B2
group, is responsible for the synthesis of the secondary metabolite colibactin (Arthur, J.C., et al. (2012), Science
338(6103), 120–123). In addition, we recently observed a correlation between the presence of hydrogen sulfide
(H2S)-producing bacteria (HSPB) and the severity of new onset Crohn's disease (CD) in a pediatric population
(Mottawea, W., et al, 2016. Nat Commun 7, 1–14.). Our long-term goal is to determine how bacteria-host
interaction influences the development of colitis-associated CRC. The objective of the present competitive
renewal is to determine how the host and environmental factors regulate the carcinogenic potential of bacteria.
The central hypothesis of this project is that host-derived signaling modulates bacterial-generated metabolites
to influence carcinogenesis. The rationale for the proposed research is that once we understand the interplay
between bacteria and the host, it would be possible to target specific activity and thus development of colitis-
associated CRC. We plan to test our central hypothesis and fulfill the overall objective of this application with the
following specific aims.
Aim 1. Define inflammatory requirement necessary for bacteria-induced CRC in Il10-/-;Apcmin/+ mice.
Aim 2. Establish temporal mechanism implicated in bacteria-induced CRC in Il10-/-;Apcmin/+ mice.
Aim 3. Determine microbial responses and CRC development following cell type-specific alteration of
of MYD88 signaling in Il10-/- mice.
 The results will potentially not only open new directions of colorectal cancer research (inflammation
modulation of microbial functions) but also provide novel targets (tissue-specific microbial sensing and
microbial disease-promoting activity) and means (anti-inflammatory and anti-microbial agents) for treating and
preventing colorectal cancer.

## Key facts

- **NIH application ID:** 10132299
- **Project number:** 5R01DK073338-14
- **Recipient organization:** UNIVERSITY OF FLORIDA
- **Principal Investigator:** Christian Jobin
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $429,377
- **Award type:** 5
- **Project period:** 2007-04-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10132299

## Citation

> US National Institutes of Health, RePORTER application 10132299, Role of Bacteria in Colitis-Associated Colon Cancer (5R01DK073338-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10132299. Licensed CC0.

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