# Core C - Proteomics Core

> **NIH NIH U19** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2020 · $322,009

## Abstract

SUMMARY 
In the Proteomics Core (Core C), part of the Dengue Human Immunology Project Consortium, we will apply 
mass spectrometry-based proteomics approaches to quantitatively measure responses to viral infection and 
vaccination in clinical cohorts. We will first measure global changes in a number of protein readouts including 
phosphorylation, ubiquitination, and protein abundance, in ex vivo primary cells infected with Dengue virus to 
identify proteins of interest worth pursuing in more targeted proteomics studies in clinical samples. Data from 
Core C will be integrated with data from Cores B (Genomics Core) and D (Immune Monitoring Core) by Core E 
(Data Analysis and Modeling Core) to select approximately 200 proteins for which to develop sensitive assays 
for detection and quantification from very limited sample amounts. Finally, we will apply the developed assays 
to quantify protein responses in patient-derived samples from clinical cohorts in natural populations to compare 
clinical outcomes of viral infection and vaccination. These targeted proteomics approach will also be used to 
conduct ex vivo validation studies after perturbing predicted host driver genes.

## Key facts

- **NIH application ID:** 10132471
- **Project number:** 4U19AI118610-06
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** Jeffrey R Johnson
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $322,009
- **Award type:** 4C
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10132471

## Citation

> US National Institutes of Health, RePORTER application 10132471, Core C - Proteomics Core (4U19AI118610-06). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10132471. Licensed CC0.

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