# Project 2 - Immune profiling of candidate vaccines for dengue

> **NIH NIH U19** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2020 · $73,549

## Abstract

Takeda Vaccines Inc., is developing a live attenuated tetravalent Dengue vaccine candidate 
(TDV) that consists of an attenuated DENV-2 strain (TDV-2) and three chimeric viruses 
containing the prM and E protein genes of DENV-1, -3 and -4 expressed in the context of the 
attenuated TDV-2 genome backbone (TDV-1, TDV-3, and TDV-4, respectively). A better 
understanding on how innate immune responses to recipients of TDV vaccine shape adaptive 
immunity is essential for the development of a safe and effective vaccine. In project 2 of the 
Dengue Human Immunology Project Consortium (DHIPC) we apply a systems biology approach 
to dissect the qualitative and quantitative features of the innate and adaptive immune responses 
generated by TDV. The overall goal of Project 2 is to identify gene expression signatures 
following TDV vaccination that will be correlated to measurements of tetravalent neutralizing 
antibody titers and/or the magnitude and multifunctionality of T cell responses. This should 
enable identifying markers predictive of vaccine immunogenicity and efficacy. The innate 
immune signatures acquired in Project 2 will be compared to those obtained in parallel 
investigations of samples from natural human DENV infections (Project 1) and ex vivo infection 
of relevant primary immune cells with the same viruses to gain mechanistic insight (Project 3).

## Key facts

- **NIH application ID:** 10132477
- **Project number:** 4U19AI118610-06
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** Jorge E Osorio
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $73,549
- **Award type:** 4C
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10132477

## Citation

> US National Institutes of Health, RePORTER application 10132477, Project 2 - Immune profiling of candidate vaccines for dengue (4U19AI118610-06). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10132477. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*