# Spatial and single cell analysis of tumor microenvironment by seqFISH+

> **NIH NIH R44** · SPATIAL GENOMICS, INC. · 2021 · $999,999

## Abstract

SUMMARY
Tumor microenvironment (TME) is a key determinant of tumor growth, disease progression and
resistance to chemotherapy. There is a diverse repertoire of distinct cell type that are found in
the tumor microenvironment, with many that have not been fully enumerated. In addition, little is
known about the “geography” of the tumors and the spatial organization of the distinct cell types.
There is therefore, an immediate need of transformative tools to enable in-depth understanding
of the TME in many forms of malignancies.
Spatial Genomics Inc is commercializing seqFISH+ (Eng et al, Nature 2019) which can achieves
multiplexing of 10,000 genes at the mRNA level in single cells in tissues with spatial information
preserved. Using the seqFISH+ transcriptome profiles, novel cells types in situ and spatial
organizations can be directly identified and visualized in tumor tissue slices.
These capabilities make seqFISH+ a powerful tool for examining the different cell types and their
interactions in the tumor microenvironment. In this SBIR proposal, we will develop an instrument
and the associated software and reagents that will allow researchers to perform seqFISH+ in
tumor slices. In collaboration with Amgen and Children’s Hospital at LA (CHLA), we will apply
seqFISH+ to image the tumor microenvironment in both mouse model systems and human
pediatric neuroblastoma samples.

## Key facts

- **NIH application ID:** 10133025
- **Project number:** 5R44CA247353-02
- **Recipient organization:** SPATIAL GENOMICS, INC.
- **Principal Investigator:** Kirsten Frieda
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $999,999
- **Award type:** 5
- **Project period:** 2020-04-01 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133025

## Citation

> US National Institutes of Health, RePORTER application 10133025, Spatial and single cell analysis of tumor microenvironment by seqFISH+ (5R44CA247353-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10133025. Licensed CC0.

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