# Supplement to DEFINING PATHWAY-SPECIFIC KINASE SIGNALING MODULES WITH PROTEOMICS

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2020 · $101,870

## Abstract

Project Summary: This proposal requests funds for a high-Field Asymmetric-waveform Ion Mobility Source
(Thermo FAIMS Pro), an accessory for our existing Thermo Orbitrap Fusion Lumos mass spectrometer which
we acquired through a NIH high-end Shared Instrument Grant (SIG). This addition would greatly enhance the
performance of our Lumos MS and would be invaluable for the successful completion of existing NIH funded
projects requiring sensitive and in-depth characterization of proteins and post-translational modifications.
Notably, several of our projects used tandem mass tagging (TMT)-based to achieve multiplexed analyses of
proteomics samples. Data from several labs, including ours, has shown that TMT-based multiplexing can lead
to quantitative errors with medium- to high-complexity samples. A recent publication describes the use of the
FAIMS Pro hardware to improve quantitative accuracy in TMT-based datasets. We propose that the acquisition
of the FAIMS Pro accessory will enable and drive the successful completion of our funded research.
Furthermore, the FAIMS Pro would also benefit existing users of the NIH SIG instrument at the University of
Washington.

## Key facts

- **NIH application ID:** 10133242
- **Project number:** 3R01GM129090-02S1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Shao-En Ong
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $101,870
- **Award type:** 3
- **Project period:** 2019-05-01 → 2023-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133242

## Citation

> US National Institutes of Health, RePORTER application 10133242, Supplement to DEFINING PATHWAY-SPECIFIC KINASE SIGNALING MODULES WITH PROTEOMICS (3R01GM129090-02S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10133242. Licensed CC0.

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