# Flow Cytometry Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2021 · $291,101

## Abstract

FLOW CYTOMETRY SHARED RESOURCE (Core-731)
ABSTRACT
Overview: The Flow Cytometry Shared Resource (FCSR) supports translational and clinical cancer research
projects of UCCC members with flow cytometric analysis, high-speed cell sorting, multiplexed fluorescent
microsphere assays, and cell counting services. Equipment: FCSR houses three high-speed sorters, four
analyzers (3,000 and 8,000 hours/year of cell sorting and analysis capacity, respectively), a new CYTOF 2 mass
cytometer, a Luminex Magpix system (1,500 hours/year capacity), and a cell counter. Services: FCSR provides
a wide range of assays particularly pertinent to cancer research, including cell cycle, cell proliferation, apoptosis,
cell viability, cell signaling, multiplexed cytokine quantitation, stem cell detection, fluorescent protein analysis,
and cell phenotyping along with cell sorting. Consultation and Education: FCSR provides consultation to help
those members with limited flow cytometry background to design appropriate experiments. In addition, regular
educational sessions are held to inform UCCC members and the investigators in their laboratories about the
fundamentals of flow cytometry along with information on new techniques and FCSR capabilities. Many
investigators also call FCSR on an ad hoc basis for advice on performing experiments. The FCSR also offers
assistance and collaboration in the development of new methods. Management: This resource is an institutional
core managed by the UCCC and is overseen by the Associate Director for Basic Research. Use of Services:
FCSR is among the most highly used of the UCCC shared resources. Since July 2011, 266 investigators have
used the services. Sixty-two percent of users (164) were UCCC members, representing all six programs and
resulting in 160 peer-reviewed publications. CCSG funding represents 19% of the annual operating budget. The
remaining support comes from a NIAMS P30 grant (1%), institutional support (4%) and user fees (75%). Future
Directions: FCSR will continue to play a central role in the research conducted by UCCC members by continuing
to provide outstanding flow cytometry related services and education and developing cross-disciplinary
collaborations with other shared resources. In addition, the FCSR has several future directions that will enhance
the SR and UCCC member cancer research including: 1) Raise awareness and promote use of CyTOF2
instrument; 2) Acquire a four- or five-laser analyzer that can detect at least 14 colors and evaluate samples from
96-well plates.

## Key facts

- **NIH application ID:** 10133535
- **Project number:** 5P30CA046934-33
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Dan Theodorescu
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $291,101
- **Award type:** 5
- **Project period:** 1997-04-04 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133535

## Citation

> US National Institutes of Health, RePORTER application 10133535, Flow Cytometry Shared Resource (5P30CA046934-33). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10133535. Licensed CC0.

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