# Functional Genomics Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2021 · $185,637

## Abstract

FUNCTIONAL GENOMICS SHARED RESOURCE (Core-564)
ABSTRACT
Overview: Functional Genomics explores gene and protein function at the individual gene level and on a
genome-wide scale. The Functional Genomics Shared Resource (FGSR) provides UCCC members access to
tools that investigate gene function on a genome-wide scale; develops novel protocols and offers expertise for
the use of Functional Genomics tools; and provides a forum for scientific exchange. By promoting collaboration
in Functional Genomics, the FGSR serves to catalyze discoveries by UCCC members. Equipment: Through
continuous assessment of novel technologies and UCCC members' needs, the FGSR has acquired diverse
tools, including genome-wide short hairpin RNA (shRNA), Open Reading Frame (ORF) and CRISPR libraries.
Today, our human shRNA collection contains 176,283 clones targeting >22,000 unique genes, while our mouse
collection contains 138,538 clones targeting >21,000 unique genes. Our ORF library contains 15,744 plasmids
for protein overexpression corresponding to 13,082 unique human genes. Our pooled gene knockout CRISPR
libraries contain an average of 6 guide RNAs (gRNAs) per gene to inactivate ~18,000 human genes and ~15,000
murine genes. The transactivating CRISPR library contains similar numbers of gRNAs to induce activation of
~18,000 human promoters. Our arrayed CRISPR library has 2 gRNAs per gene for 16,190 human genes.
Services: The FGSR assists members in all aspects required for the effective use of the tools provided,
preparing bacterial glycerol stocks, providing plasmids for entire libraries or individual clones, and preparing
lentiviral particle suspensions. The FGSR also works with client labs to create `custom panels' targeting gene
collections of interest. Consultation and Education: The FGSR personnel assist members on a daily basis with
experimental design and troubleshooting. The FGSR website hosts a large number of very detailed protocols
and bibliographic sources that are updated constantly. The FGSR also works with program leaders to host
`technology forums' where UCCC members are updated on the latest developments and applications in the field
of Functional Genomics. Management: The FGSR is managed by the UCCC, and is overseen by the Associate
Director for Basic Research. Use of Services: Since July 2011, 158 client laboratories have used the services.
Seventy-seven percent of these groups (122) were UCCC members, representing all 6 Programs and resulting
in 65 peer-reviewed publications. CCSG funding represents 41% of the annual operating budget. The remaining
support comes from user fees (59%). Future Directions: We will continue to acquire the latest tools in the
Functional Genomics field, such as novel CRISPR-based technologies for genome editing. We will also expand
our service and education portfolio via technology forums and webinars.

## Key facts

- **NIH application ID:** 10133536
- **Project number:** 5P30CA046934-33
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Dan Theodorescu
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $185,637
- **Award type:** 5
- **Project period:** 1997-04-04 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133536

## Citation

> US National Institutes of Health, RePORTER application 10133536, Functional Genomics Shared Resource (5P30CA046934-33). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10133536. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*