# Protein Production / Monoclonal Antibody / Tissue Culture Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2021 · $218,900

## Abstract

PROTEIN PRODUCTION/ MONOCLONAL ANTIBODY/ TISSUE CULTURE SHARED RESOURCE (Core-544)
ABSTRACT
Overview: The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PMTSR) supports
basic and translational research projects of the UCCC members by providing authenticated cell lines, producing
monoclonal antibodies and recombinant proteins, and providing real time live cell imaging of cultured cells.
Equipment: In 2015, we acquired an Essen BioScience IncuCyte, which offers real time imaging of living cells
to quantitate cell proliferation, apoptosis, cell migration, chemotaxis and gene expression in an automated, high
throughput manner. This instrument offers new technical advantages that were not previously available to our
investigators. Services: The PMTSR has assembled a collection of over 200 authenticated human cancer cell
lines, which are available to UCCC members at a reduced price relative to commercial services, to encourage
the responsible use of established cell lines in biomedical research. Investigators can use the PMTSR to prepare
large volumes (0.5 to 10 liters) of cells for in vitro and in vivo biological studies. For over 25 years, the PMTSR
has produced hybridomas and purified monoclonal antibodies for use in basic and preclinical studies.
Recombinant proteins are produced using baculovirus expression systems or in mammalian (293) cells.
Consultation and Education: The PMTSR has advocated for the use of authenticated cell lines in cancer
research by providing seminars outlining the impact of contaminated/misidentified cell lines upon biomedical
research and by providing information on cell authentication services to UCCC members. A number of seminars
have been offered regarding the use and technical abilities of the IncuCyte Bioimaging system. The PMTSR
director and staff routinely consult with UCCC members on projects involving offered services and technologies.
Management: The PMTSR is a UCCC SR managed by the UCCC and is overseen by the Associate Director
for Basic Research. CCSG funding represents 31% of the annual operating budget. The remaining 69% support
is from user fees. Use of Services: Since July 2011, 157 investigators have used the PMTSR services. Fifty-
seven percent of users were UCCC members, representing all UCCC Programs and resulting in 65 peer-
reviewed publications. Future Directions: 1) Expand our collection of authenticated cancer cell lines and
continue to make them available to UCCC members at reduced prices as a means to ensure compliance with
new NIH and journal policies. 2) Collaborate with Structural Biology Shared Resource (SBSR) to develop service
offering on-demand purification of recombinant proteins. 3) Support the expanded use of the IncuCyte in cancer
research. 4) Contribute to international efforts to develop platforms for murine cell line authentication and when
available, provide these authenticated lines to UCCC members.

## Key facts

- **NIH application ID:** 10133545
- **Project number:** 5P30CA046934-33
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Dan Theodorescu
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $218,900
- **Award type:** 5
- **Project period:** 1997-04-04 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133545

## Citation

> US National Institutes of Health, RePORTER application 10133545, Protein Production / Monoclonal Antibody / Tissue Culture Shared Resource (5P30CA046934-33). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10133545. Licensed CC0.

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