# Structural Biology Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2021 · $161,760

## Abstract

STRUCTURAL BIOLOGY SHARED RESOURCE (Core-405)
ABSTRACT
Overview: The Structural Biology Shared Resource (SBSR) aims to facilitate and promote the application of
macromolecular structural biology methods in cancer research for UCCC members. SBSR meets these goals
by providing UCCC members instrumentation and expertise in X-ray Crystallography (X-ray) and Nuclear
Magnetic Resonance Spectroscopy (NMR). Examples of projects studied in the SBSR fall into three main topic
areas: Dynamics of cancer target activity, gene regulation, chromatin and epigenetics, and molecular targeting
for the development of novel anti-tumor agents. Equipment: The SBSR capabilities have expanded significantly
over the last 5 years through institutional support and the NIH Shared Instrument Grant Program. As a result,
the X-ray facility has acquired a new in-house data-collection system that consists of a Rigaku MicroMax-007 X-
ray generator, PILATUS3 R 200K Hybrid Pixel Array Detector with VariMax Optics and Oxford cryo-cooling
systems. It also has a Rigaku/MSC robotics system for production of crystallization screens, drop setting and
plate imaging. The NMR facility is equipped with Agilent/Varian 500, 600 and 900 MHz spectrometers at the
Anschutz Medical Campus and an Agilent/Varian 800 MHz spectrometer at the UCB campus. Services: The
SBSR provides expertise and access to highly specialized instrumentation for X-ray crystallography and NMR
based structural studies of biomolecules relevant in cancer biology. Consultation and Education: SBSR
personnel provide advice on sample preparation, data collection, structure determination, and data presentation
for publication. Hands-on instrument training is important function of SBSR staff. Management: The SBSR is an
institutional core managed by the institution as part of the Structural Biology and Biophysics Core Facilities.
CCSG funding represents 34% of the annual operating budget. The remaining support comes from institutional
support (50%) and user fees (16%). In regard to UCCC, SBSR is overseen by the Associate Director for Basic
Research. Use of Services: Since July 2011, 54 investigators have used the services. Thirty-five percent of
users were UCCC members, representing five of the six UCCC Research Programs and resulting in 55 peer-
reviewed publications. Future Directions: The SBSR has several primary future directions that will enhance the
SR and UCCC member cancer research: 1) Outreach to UCCC members from non-structure labs to create
awareness of the opportunities SBSR technology offers and provide training in such technology; 2) incorporate
into SBSR the new Cryo-Electron Microscopy resources that are currently being developed; 3) Collaborate with
PMTSR to develop a comprehensive protein expression and purification service; 4) Initiate lifecycle replacement
of several NMR resources.

## Key facts

- **NIH application ID:** 10133547
- **Project number:** 5P30CA046934-33
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Dan Theodorescu
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $161,760
- **Award type:** 5
- **Project period:** 1997-04-04 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10133547

## Citation

> US National Institutes of Health, RePORTER application 10133547, Structural Biology Shared Resource (5P30CA046934-33). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10133547. Licensed CC0.

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