Microscopy Upgrades for three-color single-molecule studies of genome integrity

NIH RePORTER · NIH · R35 · $126,806 · view on reporter.nih.gov ↗

Abstract

Summary My group has a long–standing interest in understanding the molecular mechanisms that contribute to cancer biology and genome integrity. To provide new insights into the mechanisms of genome integrity we have pioneered the development of DNA curtains, which allow for the direct visualization of hundreds to thousands of individual molecules in real–time by optical microscopy. This experimental platform is applicable to many types of protein–nucleic acid interactions and allows us to address questions about complex biological problems that cannot be addressed through traditional approaches. Our success in with this technology has uniquely positioned the lab to reveal new concepts regarding the mechanisms of genome integrity

Key facts

NIH application ID
10134562
Project number
3R35GM118026-05S1
Recipient
COLUMBIA UNIVERSITY HEALTH SCIENCES
Principal Investigator
Eric C Greene
Activity code
R35
Funding institute
NIH
Fiscal year
2020
Award amount
$126,806
Award type
3
Project period
2016-05-01 → 2021-04-30