# Molecular Mechanisms of Membrane Disruption induced by Early-Stage Aggregation of Abeta Peptides

> **NIH NIH R01** · STATE UNIVERSITY OF NY,BINGHAMTON · 2020 · $56,712

## Abstract

Abstract
 This supplement requests a new Microwave Peptide Synthesizer for the parent project (R01-
GM125853). The parent project proposed to study the molecular basis of membrane disruptions induced by
the aggregation of -amyloid (A ) peptides. In order to achieve the research goals, a number of isotope-
labeled A peptide sequences need to be synthesized to probe specific interactions between peptides and
lipids, or to obtain molecular structural constraints to build structural models. This nature of the proposed
research places peptide synthesis in a critical position.
 Currently, all peptide syntheses are completed manually in the PI’s laboratory. The manual synthesis is
labor-intensive and time-consuming, and the crude products obtained usually contain relatively high
abundances of impurities, which further cause lower yield after purification. The required instrument utilizes
microwave, which significantly reduces the reaction time with more efficient and precise heating. By estimation,
the synthesis time per peptide sequence can be reduced to ~ 20% and the byproducts in crude peptides can
be largely eliminated. Therefore, the time allocation for peptide synthesis can be greatly shortened and the
spectrometer time, such as the time spent on solid-state nuclear magnetic resonance (ssNMR) spectroscopy
for full structural characterization, can be utilized more efficiently. For some aims, more quantitative methods
will be applied, which requires high-purity peptides. The usage of microwave-based synthesis will help to
achieve the reduction of side reactions. Overall, the requested instrument, if granted, will significantly benefit
the proposed research in parent project.
 The synthesizer will be dedicated to the PI’s research project that is funded by NIGMS. A small portion
of time, roughly 5%, will be used for other NIGMS-funded PIs who are either in the same department or
collaborators to the PI. The equipment will be operated and maintained by the PI, who has ~ 20 years’
experience in solid-phase peptide synthesis and the usage of peptide synthesizers. The Chemistry Department
at Binghamton University also provides technical support in repairing scientific instrumentations with no charge
for labors.

## Key facts

- **NIH application ID:** 10135212
- **Project number:** 3R01GM125853-03S1
- **Recipient organization:** STATE UNIVERSITY OF NY,BINGHAMTON
- **Principal Investigator:** Wei Qiang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $56,712
- **Award type:** 3
- **Project period:** 2018-01-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10135212

## Citation

> US National Institutes of Health, RePORTER application 10135212, Molecular Mechanisms of Membrane Disruption induced by Early-Stage Aggregation of Abeta Peptides (3R01GM125853-03S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10135212. Licensed CC0.

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