# Administrative Supplement: Characterizing the metabolome and volatilome of gut microbiome.

> **NIH NIH R35** · OHIO STATE UNIVERSITY · 2020 · $189,043

## Abstract

Project Summary
Previous studies have shown that microbial metabolites are often the compounds most markedly altered in the
disease state when comparing diseased versus healthy individuals. Recent studies also suggest that metabolites
deriving from microbial transformation of dietary components have significant effects on host physiological
processes such as energy metabolism, gut and immune homeostasis, neurological behavior, and vascular
function. Additionally, it has been proposed that metabolomics-directed functional interventions using exogenous
metabolites may play critical biological roles in cellular activities. However, there are no existing, detailed, and
quantitative techniques for the measurement of a broad range of endogenous microbial metabolites, nor has
there been a systematic investigation of microbial communities for their metabolic functions. To fill the existing
gap of knowledge, the research program outlined in this proposal focuses on three core areas: 1) Develop
advanced mass spectrometry-based metabolomics technique for sensitive, quantitative detection and broad
coverage of gut microbial metabolites; 2) Develop Secondary Electrospray Ionization – Field Asymmetric
Waveform Ion Mobility Spectrometry – High-Resolution Mass Spectrometry (SESI-FAIMS-HRMS) for the rapid
detection of gut microbial volatile organic compound detection; 3) Utilizing existing samples from our
collaborator’s lab to validate our metabolomics methods and elucidate the functionality of catechins microbial
metabolites in obese host. These three coherent areas center on the development and application of mass
spectrometry-based metabolomics, volatilomics and related microbial biotechnology tools to enable functional
studies of microbial metabolism and nutrition-gut microbiome-host interactions. We believe this research
program has great potential to elucidate the impact of microbial metabolites on host health and diseases, and to
provide guidelines for modulating these metabolites to realize the full therapeutic potential, and for targeting
microbial metabolism to promote human health.

## Key facts

- **NIH application ID:** 10135353
- **Project number:** 3R35GM133510-02S1
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** Jiangjiang Zhu
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $189,043
- **Award type:** 3
- **Project period:** 2019-09-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10135353

## Citation

> US National Institutes of Health, RePORTER application 10135353, Administrative Supplement: Characterizing the metabolome and volatilome of gut microbiome. (3R35GM133510-02S1). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/10135353. Licensed CC0.

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