# Coordination of high fidelity replication with mutagenic translesion synthesis

> **NIH NIH R01** · STATE UNIVERSITY OF NEW YORK AT BUFFALO · 2020 · $75,024

## Abstract

ABSTRACT
This Administrative Equipment Supplement is submitted jointly with my University at Buffalo, SUNY, Department
of Biochemistry colleague, Dr. David Fernando Estrada (R35 GM133375). This supplement request is attached
to my parent R01 grant GM130761, entitled ‘Coordination of high fidelity replication with mutagenic translesion
synthesis,’ for which I am lead PI. The purpose of this Administrative Supplement is to requested 50% of the
funds required to purchase a FortéBio OctetRed96e Bio-Layer Interferometry (BLI) instrument for rapid and
reliable kinetic analysis of macromolecular interactions in real time without the need for substrate labelling. This
instrument has several advantages over Surface Plasmon Resonance (SPR), and is desperately needed to
replace our currently unusable Reichert SPR instrument. Although our Reichert SPR instrument was functional
when the parent R01 grant was submitted, it has since become unusable. This proposal has the full support of
my School’s Dean and my Department Chair, which together are providing 50% of the total cost for the
OctetRed96e, including costs for training. This FortéBio OctetRed96e BLI instrument is essential for the goals
of the parent R01 grant as all three aims heavily rely on a rapid and reliable method for kinetic analysis of protein-
protein interactions, particularly those involving wild type and mutant partners. We recently had the opportunity
to demo the OctetRed96e in our own lab using our own proteins. During this time, we confirmed unambiguously
that it was ideally suited for analyzing the exact types of protein-protein interactions required in our parent R01
grant. Of particular note, the OctetRed96e will analyze these interactions ~64-times faster and at ~3% the costs
of our unusable Reichert SPR instrument. As a direct result of this significantly increased capacity and reduced
cost, we will be able to explore directions not possible with our currently unusable Reichert SPR instrument. In
summary, the acquisition of the FortéBio OctetRed96e will restore our ability to analyze the kinetics of discrete
protein-protein interactions at a significantly increased capacity and reduced cost. As a result, it will open up
several new research directions that were not previously possible using our Reichert SPR instrument.

## Key facts

- **NIH application ID:** 10135384
- **Project number:** 3R01GM130761-02S1
- **Recipient organization:** STATE UNIVERSITY OF NEW YORK AT BUFFALO
- **Principal Investigator:** Alba Guarne
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $75,024
- **Award type:** 3
- **Project period:** 2019-02-15 → 2022-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10135384

## Citation

> US National Institutes of Health, RePORTER application 10135384, Coordination of high fidelity replication with mutagenic translesion synthesis (3R01GM130761-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10135384. Licensed CC0.

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