# Critical periods and complement regulation in diverse CNS cell types

> **NIH NIH P50** · BOSTON CHILDREN'S HOSPITAL · 2021 · $528,130

## Abstract

PROJECT SUMMARY / ABSTRACT
Project 1
In Conte Center Project 1, we will work to understand how cells of the central nervous system regulate the
expression of complement and other immune proteins, and the extent to which this regulation is coupled to
function and dysfunction at synapses. We will pursue these questions through experiments on clinical, post
mortem, and biological samples. We will determine how the expression of C4A and C4B is regulated by allelic
variation, cell type, and upstream biology (Aim 1). We will describe at single-cell resolution how diverse CNS
cell types respond to synaptic dysfunction, utilizing the Drop-seq technology we developed to analyzed
transcriptional responses in tens of thousands of individual cells (Aim 2). Finally, we will carefully evaluate
cerebrospinal fluid as a potential reservoir of information about neural-immune interactions in the CNS (Aim 3).
Through this work, we hope to better understand how neural-immune interactions play out through gene
expression and may become visible in a clinical context.

## Key facts

- **NIH application ID:** 10136093
- **Project number:** 5P50MH112491-05
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **Principal Investigator:** Steven Andrew McCarroll
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $528,130
- **Award type:** 5
- **Project period:** 2017-05-15 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10136093

## Citation

> US National Institutes of Health, RePORTER application 10136093, Critical periods and complement regulation in diverse CNS cell types (5P50MH112491-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10136093. Licensed CC0.

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