# Point-of-Care Rapid RNA test for Zika

> **NIH NIH R44** · CROSSLIFE TECHNOLOGIES, INC. · 2021 · $999,916

## Abstract

Point-of-Care Rapid RNA test for Zika
CrossLife Technologies Inc.
Project Summary/Abstract
We aim to develop a rapid point-of-test that detects ZIKV at the point-of-need in < 30
minutes and is appropriate for non-laboratory settings and non-technical personnel. Our
proprietary test exploits a novel probe reaction chemistry that allows multiplexed
detection of DNA or RNA without sample purification, making it operable as a simple,
hand-held test. Patient samples to be input directly into the device without pre-
processing and a disposable cartridge carry out amplification of all targets and reports
visible results that can be read by eye.
We have demonstrated the feasibility of a novel and simple detection of ZIKV using
TARA (Templated Assisted Rapid Assay). The key goals of the Phase 2 project are to
1) develop and evaluate automated paper strip device and to 2) validate it using clinical
samples. We will evaluate the automated paper strip device using 200 samples from a
Navy-IRB approved protocol through our CRADA collaboration with U.S. Naval Medical
Research Center and the U.S. Naval Medical Research Unit SIX. We expect a sensitivity
(>90%) and specificity (>90%) to the qRT-PCR data on the same samples.
The successful achievement of 1) time to result < 30 min, 2) sensitivity > 90%, specificity
> 90% and LOD < 500 copies/test, 3) > 90% user feedback on ease of use need for
CLIA waiver, 4) requires no instrumentation, power source, or refrigeration of reagents,
and 5) quantitative criteria for measuring the outcome of each task are exceeds or
equivalent to the RT-PCR molecular test.

## Key facts

- **NIH application ID:** 10138148
- **Project number:** 1R44TR003549-01
- **Recipient organization:** CROSSLIFE TECHNOLOGIES, INC.
- **Principal Investigator:** HyunDae Cho
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $999,916
- **Award type:** 1
- **Project period:** 2021-01-15 → 2023-05-13

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10138148

## Citation

> US National Institutes of Health, RePORTER application 10138148, Point-of-Care Rapid RNA test for Zika (1R44TR003549-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10138148. Licensed CC0.

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