# Assays to define the impact of multiplexed perturbation and spatial context on gene regulation within single cells

> **NIH NIH F32** · UNIVERSITY OF WASHINGTON · 2021 · $33,195

## Abstract

Research Summary
Assays to Define the Impact of Multiplexed Perturbation and Spatial Context on Gene Regulation
 within Single Cells
Cells rewire gene expression in response to numerous environmental, developmental and therapeutic
stimuli, and specific responses vary greatly among individual cell types. Such genomic
reprogramming is established through DNA-binding transcription factors (TFs), which direct a
dynamic system of changes to the state of chromatin near promoters and enhancers (Takahashi and
Yamanaka 2016). However, measuring and interpreting such genomic choreography remains
extremely challenging in heterogeneous cell populations or tissue samples. To address this issue, I
describe a novel approach for multiplexed, combinatorial indexing-based, single cell ATAC-seq
(sciPlex-ATAC), which enables the simultaneous profiling of the accessible genome within thousands
of individual cells from virtually unlimited experimental conditions. I propose sciPlex-ATAC as a novel
and informative platform for characterizing perturbations of the chromatin landscape in
high-throughput chemical screens. Using this platform I plan to investigate targeted disruption of
allogeneic immune stimulation in human mixed lymphocyte reactions. Finally, by adapting the nuclear
labeling principles of sciPlex-ATAC, I envision “chromatin histology” as a method to resolve the
spatial locations of single cell chromatin profiles within tissues.

## Key facts

- **NIH application ID:** 10139376
- **Project number:** 1F32GM140502-01
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Gregory Thomas Booth
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $33,195
- **Award type:** 1
- **Project period:** 2021-06-01 → 2021-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10139376

## Citation

> US National Institutes of Health, RePORTER application 10139376, Assays to define the impact of multiplexed perturbation and spatial context on gene regulation within single cells (1F32GM140502-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10139376. Licensed CC0.

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