# TR&D Project 2. The Analysis Stage I: Tools for Analyzing the Composition and Stoichiometry of Macromolecular Assemblies

> **NIH NIH P41** · ROCKEFELLER UNIVERSITY · 2021 · $169,458

## Abstract

PROJECT SUMMARY
TR&D Project 2. The Analysis Stage I: Tools for Analyzing the Composition and Stoichiometry of
Macromolecular Assemblies
The determination and quantitation of proteins within assemblies remain a significant challenge, as proteins carry
much of the burden of the rapid dynamic responses of the cell and are incredibly diverse in their abundance and
their physico-chemical properties, making them highly versatile for the dynamic tasks at hand but at the same
time difficult to analyze. This TR&D will allow the researcher to quantitatively characterize the constitution of
isolated assemblies, and achieve this objective with a fraction of the time, effort, and resources normally required.
Thus, we will develop methods to faithfully and fully identify the bone fide components of any target
macromolecular complex in the cell, as well as that of vicinal associators, with high accuracy and throughput.
We will advance current quantitative approaches for determining complex composition. We will also provide the
community with a suite of options for discriminating between specific interactors and contaminants.We will also
develop methods to faithfully and fully identify with high accuracy the stoichiometry of any target macromolecular
complex in the cell. We will provide this technology to the community in the form of a “Stoichiometry Package”.
This package will incorporate a parallel suite of techniques that give complementary and cross-verifying
information, collectively providing accurate, comprehensive sets of stoichiometry data (and dynamic changes)
for an isolated assembly. Users can choose any or a combination of approaches, depending on need. We will
also gather data that inform on the dynamics of macromolecular complexes, by isolating complexes in defined
sequences in space and time; comparisons of how the complexes change with time or different assembly state
in terms of composition and stoichiometry will allow reconstruction of these dynamic processes. The major
challenge we are addressing here is to achieve these goals with high fidelity in a mode that is readily
transportable to any researcher.

## Key facts

- **NIH application ID:** 10140367
- **Project number:** 5P41GM109824-08
- **Recipient organization:** ROCKEFELLER UNIVERSITY
- **Principal Investigator:** JOHN D. AITCHISON
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $169,458
- **Award type:** 5
- **Project period:** 2014-08-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10140367

## Citation

> US National Institutes of Health, RePORTER application 10140367, TR&D Project 2. The Analysis Stage I: Tools for Analyzing the Composition and Stoichiometry of Macromolecular Assemblies (5P41GM109824-08). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10140367. Licensed CC0.

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