# Generation of a Cellular Atlas of Adipose Tissue in Mouse and Man

> **NIH NIH RC2** · BETH ISRAEL DEACONESS MEDICAL CENTER · 2021 · $1,991,293

## Abstract

ABSTRACT
Adipose tissue plays a critical role in regulating systemic metabolism. The adipose depots themselves
are heterogeneous mixtures of cell types that include immune cells, preadipocytes, stromal
fibroblasts, endothelial cells, and others, in addition to the adipocytes themselves. The relative
proportions of these cells, as well as their activation state, is known to differ following changes in
nutritional state. Despite a longstanding realization that the behavior of adipose tissue is determined
by interactions between the various component cell types within the fat pad, our knowledge of the
identity of these cellular sub-populations is woefully incomplete. Here we propose to use single cell
sequencing technology to identify novel subpopulations of cells within different adipose depots of mice
and humans. We will assess how these different cells vary with sex, age, and metabolic perturbations,
like fasting, high fat diet, gastric bypass, and thermal stress. Further, we will determine the activation
state of these cells under these different conditions, and will utilize a variety of experimental and
computational techniques to characterize the interactions that occur between cell types. The resulting
database will be a community resource and the starting point for a new era of adipose tissue biology.

## Key facts

- **NIH application ID:** 10141225
- **Project number:** 5RC2DK116691-04
- **Recipient organization:** BETH ISRAEL DEACONESS MEDICAL CENTER
- **Principal Investigator:** Orr Ashenberg
- **Activity code:** RC2 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,991,293
- **Award type:** 5
- **Project period:** 2018-04-23 → 2023-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10141225

## Citation

> US National Institutes of Health, RePORTER application 10141225, Generation of a Cellular Atlas of Adipose Tissue in Mouse and Man (5RC2DK116691-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10141225. Licensed CC0.

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