# COVID Immunophenotyping Study

> **NIH NIH R01** · BENAROYA RESEARCH INST AT VIRGINIA MASON · 2020 · $543,574

## Abstract

Project Summary/Abstract
This application is being submitted to PA-20-135 in accordance with NOT-AI-20-034. We are requesting an
emergency supplement to R01AI32774 “Mechanisms of IL-6 mediated T cell pathogenesis in autoimmunity”
(PI: Jane H. Buckner). This supplement will directly address the NOT-AI-20-034 research area of interest
“Identification and evaluation of the innate, cellular and humoral immune response to SARS-CoV-2
infection…..”. Importantly, the proposed work is part of the NIAID Immunophenotyping assessment in a
COVID-19 Cohort (IMPACC) study tasked with performing longitudinal immunophenotyping from 1,000
subjects with COVID-19. For each subject, among other assays, transcriptomic profiling will be done on
peripheral blood, nasal swabs and endotracheal aspirates at six different time points. Specific to the
emergency supplement requested here, we will be responsible for performing both the bulk host mRNA-
sequencing (RNA-seq) and PCR quantification of viral nucleic acid from SARS-CoV-2 for each of the ~6,000
anticipated nasal swab samples. In addition, we will perform a pilot study on 60 nasal swab samples
assessing the potential utility of metagenomic sequencing on these samples to consider it in a larger scale in
this cohort. All work will be done in close coordination with NIAID, University of California, San Francisco
(endotracheal RNA-seq), Emory University (blood RNA-seq), and Icahn School of Medicine at Mount Sinai
(virology) core sites.

## Key facts

- **NIH application ID:** 10143958
- **Project number:** 3R01AI132774-03S1
- **Recipient organization:** BENAROYA RESEARCH INST AT VIRGINIA MASON
- **Principal Investigator:** Matthew C. Altman
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $543,574
- **Award type:** 3
- **Project period:** 2020-05-04 → 2021-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10143958

## Citation

> US National Institutes of Health, RePORTER application 10143958, COVID Immunophenotyping Study (3R01AI132774-03S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10143958. Licensed CC0.

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