# Insulin Degrading Enzyme: Physiological Function and its Spatial and Activity Modulation

> **NIH NIH R01** · UNIVERSITY OF KENTUCKY · 2020 · $8,770

## Abstract

An administrative supplement to support an undergraduate summer research experience for
rising senior Sarah Sprigg is requested. Sarah will carry out some of the investigations
proposed in Aims 1 and 2 of the parent grant. Specifically, she will explore the utility of a
fluorescent fusion construct of insulin-degrading enzyme (IDE) in following enzyme localization
in cultured live cells. She will also use the construct to evaluate potential non-localizing mutants
and the proposed close interaction with insulin in endosomes. This research experience will
provide many opportunities for Sarah to enhance her scientific, communication, and career skills.
She will follow a structured protocol in designing experiments, aimed at giving her experience in
developing and testing hypotheses and gain further experience in documenting her research
activities. She will learn mammalian cell culture techniques, sophisticated light microscopy,
CRISPR/Cas9 technology, as well as the use of FRET to demonstrate the close proximity of
molecules in cells. Sarah will present her work in regular group meetings, our university's
showcase of undergraduate scholars, and the annual state undergraduate research event. In
addition, Sarah will participate in a student led journal club in the department, write up her
summer studies in the form of a manuscript, and submit her work for presentation at the
National Conference of Undergraduate Research. Finally, Sarah will register with and
participate in programs provided by our Office of Undergraduate Research, which will further
expand her contact with students interested in research and career mentors.

## Key facts

- **NIH application ID:** 10144538
- **Project number:** 3R01GM130954-01A1S1
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **Principal Investigator:** Louis Hersh Louis Hersh Louis Hersh
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $8,770
- **Award type:** 3
- **Project period:** 2019-09-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10144538

## Citation

> US National Institutes of Health, RePORTER application 10144538, Insulin Degrading Enzyme: Physiological Function and its Spatial and Activity Modulation (3R01GM130954-01A1S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10144538. Licensed CC0.

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