# Flow Cytometry Shared Resource

> **NIH NIH P30** · WISTAR INSTITUTE · 2021 · $108,591

## Abstract

PROJECT SUMMARY – FLOW CYTOMETRY SHARED RESOURCE
The Flow Cytometry Shared Resource’s (FCSR) primary goal is to provide support for the use of flow
cytometric services by Cancer Center investigators and, regionally, to the greater basic research community.
The specific aims are to: (i) provide state-of-the-art technological capability for high-quality, multi-parameter
analyses and/or sorting of different cell types from homogeneous or mixed cell populations via staff or user-
friendly operation; (ii) provide training and ongoing support in basic and advanced user-friendly operations, as
well as expertise to assist investigators in choosing reagents or instrument conditions that optimize the use of
cytometry for their experimental needs; (iii) advise and provide technical support for analysis of flow cytometry
data for publication, research presentations, and grant applications, along with management of cytometric
data. The services provided by the facility include sample acquisition and analysis, cell sorting, on-site
instrument maintenance, training, advice, and consultation. Currently, the FCSR houses a Becton-Dickinson
(BD) FACSAria II high-speed cell sorter (installed 2009), a MoFlo Astrios EQ high-speed cell sorter (2014), and
the following user-friendly analysis instruments: 2 BD LSRIIs (18-color and 14-color), a BD FACSCelesta with
a High Throughput Sampler (HTS) (12-color, 2016), and a BD FACSCalibur with a HTS. In addition, the FCSR
provides access to an Amnis ImageStreamx imaging cytometry system. The FCSR physical plant includes
additional workstations, a library of flow cytometry journals, protocol guides, and other references. Twenty-four-
hour access is available for all investigator-operated analysis instruments, reserved through an on-line
calendar system. In the last Cancer Center Support Grant budget cycle period, there were substantial
improvements to equipment and infrastructure: first, the FCSR was relocated to a single laboratory of 1,137
NSF located in the west wing of the Cancer Center with ease of access for all Cancer Center investigators;
second, the FCSR acquired a new cell sorter, a 7-laser, 32-parameter MoFlo AstriosEQ (NIH shared
instrumentation grant, 1S10 OD017998, PI, Dr. Montaner), and a 3-laser, 12-color FACSCelesta analysis
instrument (Institute-funded). During the past project period, Cancer Center members from all Programs have
extensively utilized the FCSR to accomplish pivotal research projects and increase the priority and impact of
grant applications and peer-reviewed publications. Ongoing improvements in the FCSR enable Cancer Center
members to accomplish rapid, highly automated analyses of diverse cell populations for better phenotypical
characterization of cellular subsets and their functional characterization. This is especially valuable in analyzing
populations of blood or immune-system cells, as well as expression of fluorescent protein-expressing vectors.
Cell sorting permits the sterile purificat...

## Key facts

- **NIH application ID:** 10145611
- **Project number:** 5P30CA010815-52
- **Recipient organization:** WISTAR INSTITUTE
- **Principal Investigator:** Luis J Montaner
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $108,591
- **Award type:** 5
- **Project period:** 1997-04-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10145611

## Citation

> US National Institutes of Health, RePORTER application 10145611, Flow Cytometry Shared Resource (5P30CA010815-52). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10145611. Licensed CC0.

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