# Genomics Shared Resource

> **NIH NIH P30** · WISTAR INSTITUTE · 2021 · $221,010

## Abstract

PROJECT SUMMARY – GENOMICS SHARED RESOURCE
The Genomics Shared Resource (GSR) provides state-of-the-art infrastructure and support for integrated
functional genomics studies of Cancer Center (CC) investigators. The highly trained staff and their efforts to
maintain costs without diminishing service, provides CC members with access to the latest genomic
technologies at a minimum cost. The GSR has kept pace with rapidly moving technological developments in
the field and expanded its capabilities in response to CC needs. The GSR supports a large portfolio of
applications for functional genomics research, including on-site Next-Generation (NG) sequencing, provided in
this funding cycle by the addition of an Illumina NextSeq500 system. Other new services were added in
response to input from user meetings, individual consultations and input from CC leadership. The new services
included single-cell sequencing capabilities with the purchase of a Wafergen ICell8 system, and replacement
of microarray-based gene expression analysis with NG 3’ end-seq. An amplification-free NanoString platform
was implemented to carry out targeted gene expression using custom or commercially available panels and a
NanoString RNA-Protein application was established. Gene and microRNA assays on fresh or formalin-fixed
paraffin-embedded (FFPE) human tissues are routine. The GSR has established and improved protocols and
yields for studies on small numbers of cells with limited RNA for the assessment of specific cell types. Lastly,
the GSR implemented a qPCR-based Corynebacterium bovis detection assay that allows CC members to
identify this pathogen in animal fecal and tissue samples, strengthening collaborative efforts with the Animal
Shared Resource, and established mouse and human microsatellite assays for cell line verification. During the
last CCSG budget cycle, the GSR moved into a newly renovated 1,733 NSF laboratory space in the west wing
of the Institute with ease of access to all Cancer Center investigators. Building on a collaborative data analysis
pipeline already in place with the Bioinformatics Shared Resource, the GSR successfully established an
integrated research platform that includes the Molecular Screening Shared Resource to facilitate the
identification of leads from shRNA screens to NG sequencing and Bioinformatics analysis in a productive,
multidisciplinary scientific continuum. The GSR continues to provide critical routine services including capillary
sequencing, promoter methylation studies and quantitative real-time PCR. Leveraging the rapidly increasing
affordability of sequencing costs, together with the high-quality, reliable, personalized services provided, the
GSR will continue to be a pivotal asset for CC investigators during the next CCSG budget cycle. The GSR
plans to grow efforts in functional genomics in response to faculty expansion in the areas of epigenetics,
cancer vaccines and immunology. The GSR is also critical to disease site-specific ...

## Key facts

- **NIH application ID:** 10145612
- **Project number:** 5P30CA010815-52
- **Recipient organization:** WISTAR INSTITUTE
- **Principal Investigator:** LOUISE C. SHOWE
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $221,010
- **Award type:** 5
- **Project period:** 1997-04-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10145612

## Citation

> US National Institutes of Health, RePORTER application 10145612, Genomics Shared Resource (5P30CA010815-52). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10145612. Licensed CC0.

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