# Neutral lipid metabolism during Toxoplasma infection

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 2021 · $409,375

## Abstract

SUMMARY
Toxoplasma gondii is an opportunistic protozoan parasite that poses a significant risk to AIDS/HIV patients.
Current treatments for toxoplasmosis are ineffective against the long-lived encysted stage of T. gondii. Thus,
a constant stream of new drugs based on the identification of novel drug targets is required to offer long-term
protection against cyst reactivation. Toxoplasma is an obligate intracellular parasite that multiplies in the
cytoplasm of mammalian cells within a parasitophorous vacuole that does not fuse with any host organelles.
Differentiated parasites into cyst forms (bradyzoites) are surrounded by a thick cyst wall within mammalian
cells. How these parasites acquire their nutrients from the host cell has been an intriguing question in the field.
We previously demonstrated that one mechanism developed by replicating tachyzoites to gain access to lipids
is through the recruitment and sequestration of host nutrient-filled organelles into the parasitophorous vacuole.
For example, these parasites retrieve cholesterol and sphingolipids from host endocytic organelles and Golgi-
derived vesicles internalized into the vacuole, respectively. We provide new evidence that the cyst forms also
salvage cholesterol. Present in all mammalian cells, lipid droplets represent an opportune source of neutral
lipids for a lipid scavenger such as Toxoplasma. We show that both tachyzoites and bradyzoites rely on host
lipid droplets for growth in vitro and infectivity in animals. Exogenous addition of fatty acids to the medium
stimulate lipid droplet biogenesis in tachyzoites and bradyzoites, indicating that these parasites are capable
of scavenging fatty acids that they use to esterify neutral lipids for storage in their own LD. The overall goal of
our proposal is to unravel the mechanisms of host neutral lipid uptake and storage in tachyzoites and
bradyzoites by identifying parasite effectors involved in these pathways. Specific Aim 1 will identify the parasite
effectors involved in host lipid droplet recognition and sequestration into the parasitophorous vacuole. Specific
Aim 2 will investigate the source of cholesterol for bradyzoites and the importance of enzymes synthesizing
neutral lipids for chronic infection. Specific Aim 3 will investigate the content and biological properties of the
lipid droplets in tachyzoites and bradyzoites. We will intend to open new avenues for therapeutic interventions
based on restricting the parasite’s access to major host lipid sources and blocking lipid storage pathways in
Toxoplasma.

## Key facts

- **NIH application ID:** 10148633
- **Project number:** 5R01AI138714-04
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Isabelle Coppens
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $409,375
- **Award type:** 5
- **Project period:** 2018-05-04 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10148633

## Citation

> US National Institutes of Health, RePORTER application 10148633, Neutral lipid metabolism during Toxoplasma infection (5R01AI138714-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10148633. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*