# Mitochondrial Parts, Pathways, and Pathogenesis

> **NIH NIH R35** · BROAD INSTITUTE, INC. · 2021 · $853,896

## Abstract

Mitochondrial Parts, Pathways, and Pathogenesis
ABSTRACT
Mitochondria are ancient organelles central to eukaryotic life. Defects in these organelles underlie
a spectrum of human diseases, ranging from rare inborn errors of metabolism to common age-
associated degenerative diseases. The long-term goal of our laboratory is to achieve a systems-
level understanding of this organelle that can serve as a foundation for studying mitochondria in
health and in disease. NIGMS has provided the foundational funding for our laboratory which has
enabled our ongoing efforts to systematically define the mitochondria's protein parts, organize
them into functional pathways, and identify the proteins and pathways that underlie human
disease. We have made tremendous progress, including producing MitoCarta, the most widely
used catalog of mitochondrial protein parts. We have linked dozens of previously unstudied
proteins to biological pathways and human diseases. However the protein inventory is not yet
comprehensive and moreover contains hundreds of poorly studied proteins unlinked to any
biological process in health or disease. In the next 5 years we aim to leverage emerging
technologies in proteomics, Bayesian statistics, CRISPR, and high-resolution metabolomics, and
human genetics to unveil the function of the dark corners of the mitochondrial proteome, while also
pioneering new methods that can be generalized to other pathways within the cell.

## Key facts

- **NIH application ID:** 10151616
- **Project number:** 5R35GM122455-05
- **Recipient organization:** BROAD INSTITUTE, INC.
- **Principal Investigator:** Vamsi Krishna Mootha
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $853,896
- **Award type:** 5
- **Project period:** 2017-05-29 → 2022-07-01

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10151616

## Citation

> US National Institutes of Health, RePORTER application 10151616, Mitochondrial Parts, Pathways, and Pathogenesis (5R35GM122455-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10151616. Licensed CC0.

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