# Predicting intracellular drug concentrations in the presence of transporters

> **NIH NIH R01** · TEMPLE UNIV OF THE COMMONWEALTH · 2020 · $127,776

## Abstract

We are requesting funds to upgrade our mass spectrometry capabilities. Until this year, Dr
Nagar's and Korzekwa's laboratories have used two standard resolution mass spectrometers
(Sciex API 4000s) for all LCMSMS quantitation. Although these instruments are excellent for
routine quantitation of drugs and metabolites from in vitro and plasma samples, they are not
high-resolution instruments and are not ideal for protein quantitation. Unexpectedly, we have
recently acquired a Bruker Solarix 7T FT-ICR mass spectrometer (< two years old). This
instrument has a 7-tesla magnet and is an ultra-high-resolution instrument capable of measuring
molecular masses with 1 in 2.5 million resolution. This instrument is equipped with both MALDI
and API interfaces, which allows identification and quantification of both small molecules and
proteins. In addition to exact-mass analysis and quantitation of drugs and metabolites from in
vitro and plasma samples, this instrument can perform mass spectrometry imaging, the spatial
quantitation of drugs and metabolites directly from tissues 1,2. We are requesting two upgrades
for the Bruker Solarix 7T FT-ICR mass spectrometer. First, we are requesting funds to purchase
an HTX M5 Sprayer MALDI matrix deposition system and second, an Agilent 1290 high
performance HPLC. These upgrades will be used for sample preparation and introduction into
the FT-ICR mass spectrometer through the MALDI imaging and API interfaces, respectively.
The requested upgrade to this instrument will allow us to greatly expand our analytical
capabilities and generate unanticipated datasets that will help achieve our Specific Aims
outlined in the abstract above for NIH grant 2R01GM104178 `Predicting intracellular
concentrations in the presence of transporters'.

## Key facts

- **NIH application ID:** 10154888
- **Project number:** 3R01GM104178-06S1
- **Recipient organization:** TEMPLE UNIV OF THE COMMONWEALTH
- **Principal Investigator:** Kenneth Ray Korzekwa
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $127,776
- **Award type:** 3
- **Project period:** 2013-01-15 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10154888

## Citation

> US National Institutes of Health, RePORTER application 10154888, Predicting intracellular drug concentrations in the presence of transporters (3R01GM104178-06S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10154888. Licensed CC0.

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