# MHC Genetic Typing Core

> **NIH NIH U42** · UNIVERSITY OF WASHINGTON · 2021 · $147,752

## Abstract

Project Summary/Abstract:
Macaca nemestrina serves as a critical animal model for the study and treatment of AIDS and AIDS-related
diseases. However the genomic characterization of M. nemestrina remains limited. Thus one goal of the
proposed work is to expand genome analysis, enabling the update of tools used to inform the genetic
management and assignment of M. nemestrina for S/HIV studies. Towards this goal, we have used
genotyping-by-sequencing (GBS) to identify single nucleotide polymorphisms (SNPs) with prevalent minor
alleles among subjects in the WaNPRC breeding colony. These SNPs will be used to support the design of a
96 SNP panel for use in parentage validation. The 96 SNP array will then be evaluated by genotyping a set of
96 M. nemestrina DNAs, including 6 replicate samples to test for genotype reproducibility, and 90 DNA
samples from animals with known parentage to test for accurate parentage discrimination. Once validation is
complete, the SNP array will be used to re-genotype the breeding colony, enabling a smooth transition from
the STR platform to SNP typing for parentage assessment. In addition, this project will expand MHC Class I
allele haplotype analysis of the WaNPRC breeding colony to ensure that all SPF breeding colony animals are
genetically characterized.

## Key facts

- **NIH application ID:** 10155604
- **Project number:** 5U42OD011123-17
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** CHARLOTTE HOTCHKISS
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $147,752
- **Award type:** 5
- **Project period:** 2002-09-30 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10155604

## Citation

> US National Institutes of Health, RePORTER application 10155604, MHC Genetic Typing Core (5U42OD011123-17). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10155604. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*