# Systems Approach to Immunity and Inflammation

> **NIH NIH U19** · SCRIPPS RESEARCH INSTITUTE, THE · 2020 · $366,597

## Abstract

This proposal represents a highly innovative and perhaps unique approach to lay the foundation for
understanding the pathogenesis of COVID19-driven pathologies in man. Here we bring three distinct scientific
approaches together in a novel and coordinated fashion to analyze key steps in innate immune response to
COVID-19 infection. The fundamental studies of the NLRP3 inflammasome pathway is both deep and broad
given the nearly 20-year experience of the Ulevitch lab in studying various members of the NLR family at the
level of pathway analysis. While drawing on information from studies in murine cells (genetic, biochemical and
cell biologic) the focus here is on human Mϕ where the Ulevitch lab has built a broad approach with
immunologic, cell biologic and genetic approaches. The Nolan lab has pioneered the use of high content
imaging approaches for analyzing cells and tissues. The high parameter technologies maximize the breadth of
immune features analyzed in samples for rapid discovery. CyTOF gives 42-channel info for millions of cells in
blood. CODEX spatially resolves single cells in tissues with >60 parameters, while ViralMIBI brings viral
nucleic acid detection to antibody-based multiparameter imaging defining cellular niches of infection.
Combining these technologies with SARS-CoV2 challenge models and the Collaborative Cross will also result
in the identification of mouse models that most closely match the disease phenotypes and molecular
signatures of COVID-19 disease in humans. If deemed useful we can also use information and/or murine
strains derived from forward genetic efforts in the Beutler lab. The latter has been an integral part of the
Scripps U19 program since the initial funding in 2001/2002.

## Key facts

- **NIH application ID:** 10159667
- **Project number:** 3U19AI100627-08S1
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Richard J Ulevitch
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $366,597
- **Award type:** 3
- **Project period:** 2020-06-11 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10159667

## Citation

> US National Institutes of Health, RePORTER application 10159667, Systems Approach to Immunity and Inflammation (3U19AI100627-08S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10159667. Licensed CC0.

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