# Resolution of Epithelial Cell Hyperplasia in Asthma

> **NIH NIH R01** · BRIGHAM AND WOMEN'S HOSPITAL · 2021 · $724,538

## Abstract

Project Summary/Abstract
During the previous funding period we elucidated the molecular mechanisms of Bik/Nbk-induced apoptosis in
hyperplastic airway epithelial cells (AECs) and identified Bik-derived peptides that activate Bak and reduce
mucous cell hyperplasia (MCH) in mice. While we were evaluating the effects of Bik deficiency on MCH, we
noticed that among females, but not males, bik-/- compared with bik+/+ mice show enhanced inflammation in
response to LPS and cigarette smoke exposure. Further, we noticed that instillation of Bik-derived peptides or
transgenic expression of Bik in AECs in an inducible fashion suppressed allergen-induced inflammation. Even
more striking was that IL-6 levels were increased in lung tissues of bik-/- compared with bik+/+ mice at baseline in the
absence of inflammatory stimuli, and that bik-/- mice develop emphysema at 80 weeks of age. The role of Bik in
regulating inflammation was confirmed by our observation that primary murine airway epithelial cells (MAECs) from
female bik-/- compared with bik+/+ show increased levels of nuclear factor kappaB (NF-κB). In humans, we identified
a single nucleotide polymorphism (SNP) within the BIK promoter region that is associated with decline in lung
function in four independent cohorts for subjects older than 60 years of age. The AG change causes reduced
BIK gene expression and females with reduced Bik levels show increased circulating IL-6 levels. Because Bik
blocks baseline inflammation in naïve bik-/- mice we propose that this is the actual function of Bik rather than it's cell
death inducing activity; and if left unchecked can lead to lung destruction during aging. Therefore, this renewal
application is focused on elucidating the central role of Bik in blocking inflammation by reducing nuclear p65 levels.
Aim 1 will determine the importance of localization of Bik and Bcl-2 at the ER for reducing inflammation at baseline
in the absence of inflammatory stimuli. Mutations of functional sites within Bik and Bcl-2 proteins will show the sites
of interaction between Bik, Bcl-2 and p65. Further, the possible role of increased Bcl-2 expression in female cells
causing the sex-dependent differences in inflammation will be explored. Aim 2 will test the causal role of Bik
deficiency on increased inflammation by restoring Bik levels using adenoviral expression of Bik or treating with the
Bik-derived peptide to reduce secreted IL-6 levels in differentiated bik-/- MAECs and primary human airway epithelial
cells homozygous for the G allele. Further, the causal role of Bik in the development of emphysema in aging
female mice will be evaluated using transgenic inducible expression of Bik in the lungs of bik-/- mice to block
baseline inflammation. These studies will lay the foundation for a precision medicine-based treatment of COPD and
asthmatic bronchitis.

## Key facts

- **NIH application ID:** 10162636
- **Project number:** 5R01HL068111-18
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** Yohannes Tesfaigzi
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $724,538
- **Award type:** 5
- **Project period:** 2003-07-01 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10162636

## Citation

> US National Institutes of Health, RePORTER application 10162636, Resolution of Epithelial Cell Hyperplasia in Asthma (5R01HL068111-18). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10162636. Licensed CC0.

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