# Vanderbilt O'Brien Kidney Center-Core A Physiology-Pathiophysiology Core

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2021 · $316,000

## Abstract

ABSTRACT
The Phenotyping and Pathophysiology Core is designed to address the need for effective phenotyping of
kidney function in mouse models of selective gene overexpression or deletion. In addition, this Core is
designed to provide experimental mouse models of acute or progressive renal injury to assess potential
therapeutic interventions. This Core also helps investigators who may have developed genetic mouse models
or potential therapeutic interventions but do not have the expertise and/or infrastructure required to design and
carry out appropriate kidney phenotyping studies or studies investigating mouse models of kidney injury. In this
proposal, we propose five distinct subcores that will offer and/or develop methodologies to define the
pathogenesis of kidney disease and design therapeutic interventions. The Phenotyping Subcore will provide
a battery of well-established invasive and non-invasive tests of kidney function. The Injury Model Subcore will
provide investigators cost-effective testing of potential pharmaceutical or immunologic therapy strategies in
appropriate mouse models of acute or progressive kidney injury. In addition, this subcore will provide murine
models for screening of potential kidney side effects of drugs. This subcore provides a wide variety of
experimental models of mouse kidney injury that offer consistent and reproducible results. Some of the mouse
models available have been developed by the Center investigators. The Metabolism and Bioenergetics
Subcore will offer direct measurements of kidney tissue partial oxygen consumption and will develop
methodology for the measurements of whole kidney oxygen consumption based on kidney blood flow and
measurements of arterial and renal venous partial oxygen. Furthermore this subcore will provide expertise in
metabolic flux analysis, glucose uptake and quantification of various metabolites in kidney tissues using mass
spectrometry methods. The Mouse Kidney Imaging Subcore will provide established non-invasive imaging
techniques for the assessments of mouse renal diseases and will provide methods and further optimize novel
methods for the noninvasive characterization of renal microstructure and function in mice. The CRISPR/cas9
Subcore will provide guidance for the design, construction, and generation of a CRISPR mouse.
In summary, the Phenotyping and Pathophysiology Core provides a broad range of services that aid
researchers interested in utilizing murine models for kidney-related research. The structure of this Core is
unique in that it performs a variety of kidney functions, non-invasive quantitative kidney images analysis, and
experimental platforms for the testing of drugs and interventions. Importantly, this Core performs all these
services in house and directly oversees these studies. We have been very successful in providing these
services not only to the local scientific community interested in kidney research, but also in serving as a
national and international res...

## Key facts

- **NIH application ID:** 10163164
- **Project number:** 5P30DK114809-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** Mingzhi Zhang
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $316,000
- **Award type:** 5
- **Project period:** 2017-09-08 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10163164

## Citation

> US National Institutes of Health, RePORTER application 10163164, Vanderbilt O'Brien Kidney Center-Core A Physiology-Pathiophysiology Core (5P30DK114809-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10163164. Licensed CC0.

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