# Vanderbilt O'Brien Kidney Center-Core B Histology and Molecular Pathology Core

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2021 · $142,200

## Abstract

Abstract
The goal of the Histology and Molecular Pathology Core is to provide a common facility and
expertise for the broad spectrum of morphologic studies that may be required for interpretation of
studies of various models of kidney disease and their response to interventions. Additional major
functions of this Core include providing tissue samples from existing archival specimens from
various kidney disease models that may be used for exploration and characterization of expression
patterns of various novel target molecules (tissue microarrays). The Core also aims to develop
approaches and enhance existing morphologic techniques to improve the ability of center
investigators to localize specific molecules in tissue sections, and to interpret their significance
based on the spatial localization and relationship to patterns of injury (e.g. mass spectrometry
imaging). The principal morphologic techniques provided by the Core include routine histology,
including special stains, and selected immunohistochemistry stains and novel RNA scope in situ
hydridization. In addition, electron microscopic analysis and selected morphometric techniques,
including glomerular size, tubulointerstitial fibrosis, GBM width and foot process effacement are
provided. Laser capture microscopic dissection for special studies of selected renal structures is
also offered, with mass spectrometry and/or PCR for molecules of interest in the selected regions.
Confocal Z section imaging of selected renal structures for detailed spatial analysis can also be
performed by the Core. We already have successfully developed mouse and rat kidney tissue array
samples from a wide range of experimental models for assessment of key novel molecules and their
response to interventions. We have begun development of in situ mass spectroscopy techniques to
optimize spatial resolution of mass spectra in kidney tissues, and propose to fully develop this
technique for identification of key molecules in kidney diseases. Thus, the Core will continue to offer
both state-of-the-art and standard morphologic assessments, together with development of novel
approaches to assess specific molecules in tissue sections, and to interpret their significance based
on the spatial localization and relationship to patterns of injury.

## Key facts

- **NIH application ID:** 10163167
- **Project number:** 5P30DK114809-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** AGNES B. FOGO
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $142,200
- **Award type:** 5
- **Project period:** 2017-09-08 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10163167

## Citation

> US National Institutes of Health, RePORTER application 10163167, Vanderbilt O'Brien Kidney Center-Core B Histology and Molecular Pathology Core (5P30DK114809-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10163167. Licensed CC0.

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