# Vanderbilt O'Brien Kidney Center - Core C Cell and Genome Engineering

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2021 · $142,200

## Abstract

Abstract
The cell and genome engineering core is structured to complement mouse and human in vivo studies
by genetically manipulating cells both in vivo and in vitro as well as isolating and propagating specific
renal cells. These services will be provided by a staff that is uniquely experienced with these
methodologies.
The Core will provide the following services:
1) Provide expertise with regards to genome engineering to the Nephrology community, specifically
with respect to providing CRISPR/Cas9 and transposon vectors and expertise in using them for
stable genetic modification of kidney cells in culture.
2) Isolate discrete cell populations (e.g. podocytes, proximal tubule cells) from mice that can be
cultured as either primary or immortalized cells for future use. In addition, it will isolate protein and/or
mRNA from cell-specific components of injured mouse kidneys for in depth analysis.
3) Isolate podocytes and proximal tubule cells from normal and diseased human kidneys that will be
processed for transcriptomic and proteomic analyses. The patient clinical data will be linked to the
samples via electronic records.

## Key facts

- **NIH application ID:** 10163168
- **Project number:** 5P30DK114809-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** ROY ZENT
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $142,200
- **Award type:** 5
- **Project period:** 2017-09-08 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10163168

## Citation

> US National Institutes of Health, RePORTER application 10163168, Vanderbilt O'Brien Kidney Center - Core C Cell and Genome Engineering (5P30DK114809-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10163168. Licensed CC0.

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