# Mechanisms of Motion Detection in Retinal Neural Network

> **NIH NIH R01** · WAYNE STATE UNIVERSITY · 2021 · $484,664

## Abstract

The long term goal of this research project is to elucidate the functions of neural network in the retina. A key
function of the retina is to encode distinct components of the image world, such as color and motion, by
multiple neural networks. Direction selective ganglion cells (DSGCs) play a major role in motion detection
because they respond to an object as it moves in a particular direction. Starburst amacrine cells (SACs)
contribute to direction selectivity in DSGCs by releasing GABA onto a DSGC only when an object moves from
its proximal to distal dendrites, but not when an object moves in the opposite direction, resulting in
unidirectional DSGC excitation. While the classic Barlow-Levick model remains the fundamental explanation of
direction selectivity, this model does not explain acetylcholine release from SACs and heterologous synaptic
connections between bipolar cells and SACs. Using patch clamp recordings together with morphological and
molecular biological approaches, we have investigated the temporal properties of individual bipolar and
ganglion cells and correlated neural circuits coding components of the image world. We recently found that
type 2 and 7 bipolar cells express bungarotoxin-sensitive, α7 acetylcholine receptors (α7AChRs) and
depolarize in response to a puff application of a α7AChR agonist. Because these bipolar cells provide synaptic
inputs to SACs at their proximal dendrites, we propose a new model of direction selectivity: when an object
moves from proximal to distal dendrites of SACs, the type 2 and 7 bipolar cells are activated through α7AChR
signaling and boost the excitation in SACs (preferred direction for SACs). In contrast, activation of these
bipolar cells is delayed by an object moving in the opposite direction, which in turn provides less excitation to
SACs (null direction for SACs). We will explore this model as follows: we will investigate the types of bipolar
cells that express α7AChRs using immunohistochemistry and patch clamp recordings (Aim 1). Then, we will
generate a transgenic mouse in which α7AChRs are eliminated from bipolar cells. Using this mouse, we will
test whether the direction selectivity of SACs is reduced (Aim 2). Finally, we will test whether the direction
selectivity of downstream neurons is reduced by α7AChRs elimination from bipolar cells (Aim 3). The results of
this study will increase our understanding of neural mechanisms of motion detection.

## Key facts

- **NIH application ID:** 10164792
- **Project number:** 5R01EY028915-04
- **Recipient organization:** WAYNE STATE UNIVERSITY
- **Principal Investigator:** Tomomi Ichinose
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $484,664
- **Award type:** 5
- **Project period:** 2018-06-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10164792

## Citation

> US National Institutes of Health, RePORTER application 10164792, Mechanisms of Motion Detection in Retinal Neural Network (5R01EY028915-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10164792. Licensed CC0.

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