# Transgenic Core

> **NIH NIH P30** · BOSTON MEDICAL CENTER · 2021 · $256,910

## Abstract

The BNORC Transgenic Core (Bradford Lowell, Director) is a fee-for-service facility that takes investigator-
derived DNA constructs or investigator derived (or obtained) genetically modified embryonic stem (ES) cells to
create founder transgenic and gene knockout or gene knockin mice (i.e. mice bearing point mutations, lox-
modified or ires/2A-Cre knockin alleles) that can be used to address questions relevant to diabetes, obesity,
and nutrition, including questions related to brain control of feeding, metabolism, and hunger / reward. The
Transgenic Core is heavily utilized. In the last 4 year cycle (2012-2016), the Core performed 188 projects (27
projects involving transgenic DNA injection and the injection of CRISPR/Cas9 reagents, 59 projects involving
generation of targeted ES cell clones, 83 projects involving blastocyst injection of targeted ES cell clones, and
19 projects involving cryopreservation and in vitro fertilization). This number of projects performed during this
shortened 4 year cycle represents a continued marked increase in the number of projects over prior 5 year
cycles ending in 1997 (54 projects), 2002 (91 projects), 2007 (120 projects) and 2012 (162 projects). This
demonstrates progressively increasing, substantial demand for BNORC Transgenic Core services.
Importantly, mice generated by the Core have had a major impact on science. In the last 4 year cycle (2012-
2016), BNORC Transgenic Core-generated mice have been featured in 389 publications (124 authored by
BNORC Core members and 265 authored by Non-BNORC members – the latter demonstrating extensive
sharing of BNORC Transgenic Core-generated mice with other labs). 53 of these publications were in Cell,
Nature and Science, and 55 were in Cell Metabolism, Nature Neuroscience and Neuron. With regards to
development of new technology, the BNORC Transgenic Core has implemented CRISPR/Cas9-related
approaches to engineer the mouse genome. This will further expand usage by BNORC members.

## Key facts

- **NIH application ID:** 10166828
- **Project number:** 5P30DK046200-29
- **Recipient organization:** BOSTON MEDICAL CENTER
- **Principal Investigator:** BRADFORD B LOWELL
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $256,910
- **Award type:** 5
- **Project period:** 1997-09-30 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10166828

## Citation

> US National Institutes of Health, RePORTER application 10166828, Transgenic Core (5P30DK046200-29). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10166828. Licensed CC0.

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