# Project 3 - Ex vivo immune profiling of dengue viruses and vaccines

> **NIH NIH U19** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2020 · $5,198,360

## Abstract

Through comparative analysis, different coronaviruses, including the novel SARS-CoV-2 and
human seasonal coronaviruses hCoV-OC43 and hCoV-NL63 will be used to unveil both their
similarities and differences in cellular susceptibility and permissiveness, innate immune
responses, and immune modulatory potential in primary human cell systems including monocyte
derived dendritic cells (MDDCs), normal human bronchial epithelial cells (NHBEs), and an ex
vivo tonsil histoculture (HC) system. We will use state of the art techniques to analyze those
responses intracellularly and extracellularly. Our group specializes in the manipulation of
primary human cell systems and investigating both innate immune responses to viral infection
as well as viral antagonism of innate immune sensing. As such we are uniquely positioned to
respond to this new public health threat and provide critical information regarding the molecular
biology of SARS-CoV-2.

## Key facts

- **NIH application ID:** 10167061
- **Project number:** 3U19AI118610-06S1
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** Ana Fernandez-Sesma
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $5,198,360
- **Award type:** 3
- **Project period:** 2020-06-05 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10167061

## Citation

> US National Institutes of Health, RePORTER application 10167061, Project 3 - Ex vivo immune profiling of dengue viruses and vaccines (3U19AI118610-06S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10167061. Licensed CC0.

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