# Tool and Technology Development Core

> **NIH NIH U19** · COLD SPRING HARBOR LABORATORY · 2021 · $1,404,873

## Abstract

TECHNOLOGY CORE SUMMARY
The two primary goals of this Center are to build a whole brain transcriptome atlas and a more detailed
forebrain cell anatomy atlas. These goals will be achieved by three Research Segments that will be supported
and enhanced by transcriptomic technologies and pipelines developed and refined in the Technology Core.
The Core's functions will be managed by the lead Dr. Anthony Zador (CSHL), and supported by co-leads Drs.
Evan Macosko (Broad Institute) and Edward Boyden (MIT). First, we will optimize and integrate MAPseq and
Drop-seq for high-throughput projection mapping of molecular classified neurons. MAPseq (Multiplexed
Analysis of Projections by Sequencing) is a novel technology developed by the Zador laboratory with the
capacity to determine the long-range projections of thousands or even millions of single neurons in a single
experiment. The combination of MAPseq and Drop-seq has the potential to relate projection patterns with
transcriptomes at an unprecedented scale. Second, we will integrate MAPseq with in situ sequencing to
determine the long-range projections of neurons whose spatial position and molecular identity is precisely
determined. This technique offers significantly improved spatial resolution over using MAPseq alone, and will
enable rapid and affordable brain-wide survey of gene expression and projection with high spatial resolution.
Optimization of the throughput, resolution, and cost efficiency of these techniques in the Technology Core will
complement and enhance the goals of this Center and those of the BRAIN initiative cell census network to
define the composition of the mammalian brain at the cellular level.

## Key facts

- **NIH application ID:** 10168623
- **Project number:** 5U19MH114821-05
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** ANTHONY M ZADOR
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,404,873
- **Award type:** 5
- **Project period:** 2017-09-21 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10168623

## Citation

> US National Institutes of Health, RePORTER application 10168623, Tool and Technology Development Core (5U19MH114821-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10168623. Licensed CC0.

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