The Flow Cytometry Core (Flow) offers state-of-the-art instrumentation and consultation to cancer researchers. It also provides investigators with the current and effective data analysis software as well as secure data storage options. Basic and translational cancer research projects being conducted by Holden Comprehensive Cancer Center (HCCC) members take advantage of Flow's high-speed sorting, multi-parameter analysis, high efficiency purification of cell subsets. New equipment includes two five-laser Cytek Aurora spectral analyzers with a 96- well autosamplers that can perform up to 40 color analysis and cell sorting. Flow also offers training and assists investigators with software programs available for interpretation and analysis of data. Individual training of investigators and their laboratory personnel in use of the bench-top instruments is provided which allows trained personnel access to these instruments around the clock. Flow is in a constant process of developing new technology and services as requested and/or needed by HCCC investigators.