# HIV modulation of BAG3 impacting quality control of Tau in neuronal cells

> **NIH NIH R01** · TEMPLE UNIV OF THE COMMONWEALTH · 2021 · $396,250

## Abstract

PROJECT SUMMARY
It is estimated that more than 10% of the 1.1 million HIV positive patients in United States will be over 65 years
old in less than ten years, a critical stage in life where the the risk for developing Alzheimer’s disease (AD)
increases. Earlier results from clinical studies showed several abnormalities including difficulties with memory,
thinking, and reasoning become more common in older HIV positive patients, implying that their risk factors may
be elevated when compared with their HIV negative counterparts. This notion brings up the concept that HIV
infection, even in patients whose virus is well controlled by antiretroviral therapy (ART), may be more prone to
developing neurodegenerative disorders including AD. Considering the inability of ART to effectively suppress
expression of viral proteins, including HIV Tat, which is a profoundly neurotoxic protein, we envision a scenario
in which, by dysregulating the protein quality control (PQC) pathway, Tat may perturb homeostasis of key
proteins associated with the pathogenesis of AD and set the stage for the development of disease. Several data
from our and other laboratories support this concept. First, we demonstrated that Tat inhibits expression of BAG3,
a co-chaperone/partner of HSP70 that is involved in the removal of dysfunctional and obsolete organelles
including mitochondria through a process called mitophagy, and participates in autophagy and clearance of
damaged and misfolded proteins by the protein quality control (PQC) pathway. Second, activation of BAG3 is
concurrent with a decrease in the level of phosph-tau and an increase in the clearance of tau in neuronal cells.
Third, soon after destabilization of microtubules, tau associates with Hsp70/Hsc70, a key partner of BAG3.
Fourth, induction of Tat in the brains of transgenic animals increases accumulation of phospho-tau. Thus, Tat-
mediated reduction in the level of BAG3 may have a damaging effect on neuronal cells by interrupting the
process that ensures intracellular removal of the toxic from of tau, yet maintains the healthy species of tau that
is critical for neuronal cell function. Hyper-phosphorylation of tau, which contributes to its misfolding and toxicity,
may be attributed to Tat via induction of ROS, ER stress, and mitochondrial dysfunctionality. The latter is of
particular interest as truncated tau has been implicated in dysregulation of mitochondrial dynamics and healty
energy metabolism. These observations prompted us to hypothesize that, on one hand, Tat impacts the quality
and concentration of proper levels of functional tau and the clearance of its toxic form by suppressing expression
of BAG3, and on the other hand, Tat contributes to the generation of the toxic tau and dysfunctionality of
bioenergetic pathways and mitochondria by inducing stress conditions in cells. To examine this model, we will
employ in vitro primary neuronal cultures, ex vivo animal brain tissue, and an in vivo animal model to un...

## Key facts

- **NIH application ID:** 10170194
- **Project number:** 5R01AG060962-04
- **Recipient organization:** TEMPLE UNIV OF THE COMMONWEALTH
- **Principal Investigator:** Kamel Khalili
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $396,250
- **Award type:** 5
- **Project period:** 2018-09-30 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10170194

## Citation

> US National Institutes of Health, RePORTER application 10170194, HIV modulation of BAG3 impacting quality control of Tau in neuronal cells (5R01AG060962-04). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10170194. Licensed CC0.

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