# PGC-1alpha and Reelin: new players in HAND progression.

> **NIH NIH R01** · TEMPLE UNIV OF THE COMMONWEALTH · 2021 · $396,250

## Abstract

SUMMARY
 It has been shown that patients carrying HIV-1 accumulate damage to cells and tissues that are not directly
infected by the virus itself (e.g. neurons). Importantly, these include changes known as HIV- Associated
Neurodegenerative Disorder (HAND) leading to the loss of neuronal functions.
 HAND is an outstanding problem in the clinical management of HIV-1 patients, because suppression of
infectious virus by c-ART does not completely block neurodegenerative changes. Neuropsychological studies
disclose cognitive alteration (such as loss of Spatial and Declarative Memory) in a substantial proportion of
HIV-1 infected patients, and analysis of post-mortem brain tissues isolated from HIV-1 patients treated with c-
ART show signs of neurodegeneration.
 In the absence of HIV-1 infection of neurons, a number of mechanisms have been proposed for HAND,
including indirect inflammatory effects in the CNS and direct effects of viral proteins (e.g. Tat) shed from
activated HIV-1-infected cells. The fact that these viral proteins enter the neurons through several pathways
suggest the presence of many competing mechanisms that can contribute to HAND, each of which has its
advocates. Their relative contributions to clinical disease in vivo remain to be sorted out, and this is an
outstanding problem in HIV research.
 Studies from other neurodegenerative diseases described the cAMP responsive-element binding (CREB)-1
protein as a Key Regulator of the Memory. These studies also showed that loss of CREB protein expression
and phosphorylation contributes to the development of neurocognitive impairments such as Spatial and
Declarative Memory Alteration.
 Recently, we have obtained data showing a clear-cut effect of HIV-1 Tat on CREB protein phosphorylation
and function in vitro (primary human and mouse neurons and in the neuronal cell line, SH-SY5Y) and in vivo
(in mice injected with Tat then treated with Rolipram [activator of CREB]) (see BACKGROUND AND
PRELIMINARY RESULTS). This finding is exciting for two reasons:
 (1) A correlation between CREB protein and neurodegeneration has been well-established in the literature,
 suggesting that this may be a significant contributory mechanism in HAND; and
 (2) Agents are available which can specifically restore CREB protein levels and function, offering an
 opportunity to test directly the contribution of this mechanism in vivo in mice.
 Our hypothesis is that Tat contributes to neurodegeneration via reduction of mitochondrial function,
mediated by a decrease in CREB protein activity.
 We propose to test this hypothesis in tissue culture cells and in vivo in mice, and verify the intermediate
role of CREB protein using the specific CREB activator: Rolipram.
 Therefore, in this revised application, we asked more specific and focus questions that will allow us to
perform additional in vitro and in vivo studies to determine the exact mechanisms used by Tat leading to the
loss of Memory by focusing mainly on the Tat-CREB...

## Key facts

- **NIH application ID:** 10172814
- **Project number:** 5R01AG054411-05
- **Recipient organization:** TEMPLE UNIV OF THE COMMONWEALTH
- **Principal Investigator:** BASSEL E SAWAYA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $396,250
- **Award type:** 5
- **Project period:** 2017-09-30 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10172814

## Citation

> US National Institutes of Health, RePORTER application 10172814, PGC-1alpha and Reelin: new players in HAND progression. (5R01AG054411-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10172814. Licensed CC0.

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