# Quantitative Adductomics Approaches for Assessing the Occurrence and Repair of DNA Adducts

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA RIVERSIDE · 2021 · $355,706

## Abstract

The long-term objective of this application is to discover and characterize the adductomics-based exposure
indicators for the assessment of cancer risks and for cancer prevention. Endogenous metabolism and
environmental exposure can both give rise to DNA damage. If left unrepaired, the resulting DNA adducts may
compromise the flow of genetic information by inhibiting DNA replication and transcription and inducing mutations
in these processes. In addition, the ultimate levels of DNA adducts accumulated in mammalian cells and tissues
are the result of a dynamic interplay between DNA adduct formation and repair. Thus, it is important to establish
a robust analytical method for the quantitative measurement of a broad range of DNA adducts that are implicated
in the etiology for the development of cancer and other human diseases. Such a method will also enable the
characterizations of the repair of DNA adducts, which may lead to the discovery of risk factors for cancer initiation
and development, and guide the development of approaches for effective cancer chemoprevention. In this
application, we propose to establish a DNA adductomic approach by employing and expanding our recently
established LC-MS/MS methods for the quantification of DNA adducts induced by reactive oxygen species, DNA
photoproducts arising from UV irradiation, and DNA epigenetic marks, which represent a substantial subset of
the DNA adductome. We will then employ this adductomic approach for investigating the modulations of the
levels of oxidatively induced DNA lesions and DNA epigenetic marks by DNA repair enzymes, for assessing the
implications of DNA adducts in the etiology of melanoma development, and for evaluating the effects of
sunscreen components on altering UV-induced DNA adduct formation. The proposed research will have a long-
lasting impact on the fields of DNA damage repair and cancer biology by offering a facile adductomic platform
for characterizing the risk factors and therapeutic/preventive approaches that modulate the formation and
removal of DNA adducts.

## Key facts

- **NIH application ID:** 10172860
- **Project number:** 5R01CA210072-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA RIVERSIDE
- **Principal Investigator:** Yinsheng Wang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $355,706
- **Award type:** 5
- **Project period:** 2017-06-16 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10172860

## Citation

> US National Institutes of Health, RePORTER application 10172860, Quantitative Adductomics Approaches for Assessing the Occurrence and Repair of DNA Adducts (5R01CA210072-05). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10172860. Licensed CC0.

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