This proposal requests funding for an NIH Training Program in Molecular Biophysics (the MBTP) at the University of Wisconsin-Madison. The MBTP trainees are a highly sought cohort of graduate students combining strength in mathematics and physical science with a genuine interest in complex biological systems. As students in our Biopyhysics Graduate Degree Program, MBTP trainees have a shared learning experience and benefit from a cohesive and collaborative scientific community. The selection and recruitment processes place special emphasis on recruiting students from diverse groups and have demonstrated marked improvement on this metric. The BGDP has 44 affiliated trainer labs that cover most areas of experimental and computational biophysics. These labs are spread among multiple departments and colleges, enabling the trainees to draw on a broad range of scientific input, and explore a broad range of opportunities for research. Our current trainees have a mean GRE Quantitative percentile rank of 79. The key elements in the training program are core coursework that ensures that all trainees have a firm grounding in physical and biological principles;; strong, interdisciplinary research at the forefront of molecular biophysics;; rigorous training in quantitative approaches, experimental design, and biophysical techniques;; coursework and informal training in responsible conduct of scientific research;; and close interaction with a thesis/mentoring committee that is formed in the second year and maintains a strong connection with the trainee throughout the Ph.D. program. The UW-Madison offers a tremendous range of seminars, poster sessions, and auxiliary coursework that greatly enhance the scientific breadth of our trainees. A high ratio of our trainees complete their Ph.D. training, with productive output measured by publications arising from their research and progression to their next positions in academia, industry and other professions requiring a solid scientific background. To continue to train scientists with a strong foundation in molecular biophysics, we are requesting support for six predoctoral training positions.