# Special Projects:  Method Development and Method Validation

> **NIH FDA U19** · FLORIDA STATE DEPT OF AGRIC/CONSUM SRVCS · 2020 · $35,000

## Abstract

Discipline D: Special Projects 
Analytical Track 3: Method Development and Method Validation 
Project Summary/Abstract: 
The Florida Department of Agriculture and Consumer Services (FDACS), Division of Food Safety 
Laboratories achieved accreditation in 2007 and maintains A2LA accreditation to ISO/IEC 
17025:2017 for the specific tests listed in A2LA certificates 2534.01, 2534.02 and 2534.03 
(available at www.A2LA.org). The Division has the responsibility for carrying out its duties or 
support functions as directed by the following Florida Statutes Chapters: 500, 502, 583, 586, and 
601. The Division of Food Safety is responsible for assuring the public of a safe, wholesome and 
properly represented food supply. This is accomplished through the permitting and inspection of 
food establishments; inspection and evaluation of food and dairy products; and the performance 
of biological and chemical laboratory testing on a variety of food and dairy products. 
The Division of Food Safety Laboratories are applying for the Funding Opportunity titled: 
Laboratory Flexible Funding Model (LFFM), announcement number PAR-20-105, with 
anticipation of funding from 2020 through 2025 for enhancing the capacity and capabilities of 
method development and method validation in the areas of Microbiology and Chemistry. The 
Laboratories are currently funded under the Florida Microbiology and Chemistry FDA FERN 
Program, 2015 – 2020 (5U18FD005506-05). The DFS-Bio Microbiology program has been an 
active funded laboratory since the inception on FERN microbiology program in 2004 and has 
participated in microbiology method development and method validation for FERN and the FDA. 
DFS-Bio has undertaken large-scale method development projects including the development of 
a Shiga-toxin producing E. coli (STEC) real-time PCR testing method for both O157 and non- 
O157 “Big Six” E. coli, and the development of a new method to test for Cyclospora cayetanensis 
in fresh herbs. Method development and method validation has been targeted to best suit the 
food testing needs of both the State of Florida and the nation through FDA and FERN direction. 
The Division of Food Safety Laboratories are willing and able to participate in this opportunity and 
are fully committed to increase the analytical capability and capacity of our nation’s food testing 
laboratories so as to protect the nation’s food supply through food defense and food safety 
surveillance activities, and thereby enhance efforts to protect the food supply and work toward 
ensuring the public’s health.

## Key facts

- **NIH application ID:** 10173003
- **Project number:** 1U19FD007090-01
- **Recipient organization:** FLORIDA STATE DEPT OF AGRIC/CONSUM SRVCS
- **Principal Investigator:** Jason Crowe
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** FDA
- **Fiscal year:** 2020
- **Award amount:** $35,000
- **Award type:** 1
- **Project period:** 2020-09-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10173003

## Citation

> US National Institutes of Health, RePORTER application 10173003, Special Projects:  Method Development and Method Validation (1U19FD007090-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10173003. Licensed CC0.

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